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Prostaglandin endoperoxide synthase-1 and synthase-2 expression in differentiating human monocytic cells


Prostaglandin endoperoxide synthase-1 and synthase-2 expression in differentiating human monocytic cells



Agents and Actions 41(SPEC CONF ISSUE AUG ): C159-C161



ISSN/ISBN: 0065-4299

DOI: 10.1007/bf01987621

Upon treatment with the phorbol ester TPA human myeloid leukemia U-937 cells acquire a macrophage-like phenotype including the capacity to metabolize arachidonate to prostanoids. We investigated the differentiation-associated expression of the key enzyme of prostanoid synthesis from arachidonate. prostaglandin endoperoxide synthase (PGHS). Our results of Western and Northern blot analyses indicate that the previously found 5-10-fold induction of PGHS activity during differentiation is due to enhanced expression of both currently known PGHS isoforms. In this system PGHS-2 differs from PGHS-1 in its time course of induction, its sensitivity to glucocorticoid suppression and its more extensive and heterogeneous glycosylation.

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Accession: 009262515

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