Pulsed-field gel electrophoretic discrimination of Campylobacter lari from two other thermophilic species of Campylobacter (Campylobacter coli and Campylobacter jejuni)

Matsuda, M.; Samata, T.; Imai, Y.; Kikuchi, M.; Matsumoto, K.; Kaneuchi, C.

Physico Chemical Biology 37(2): 63-67

1993


Accession: 009275394

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Abstract
Pulsed-field gel electrophoretic profiles of the respective, undigested and intact chromosomal DNAs, prepared from three species of thermophilic Campylobacter (C. coli, C. jejuni and C. lari) demonstrated that the chromosomal DNAs from C. coli JCM 2529-2, four isolated strains of C. coli, C. jejuni JCM 2013 and four isolated strains of C. jejuni migrated to around 1,900 kb, and the chromosomal DNAS from C. lari JCM 2530-2 migrated to around 1,640 kb. Chromosomal DNAs from 15 isolated strains of C. lari also migrated to almost the same extent as the DNAs from the C. lari type strain to around 1,640 kb. This result clearly demonstrates that pulsed-field gel electrophoresis (PFGE) is useful for the discrimination of C. lari from two other thermophilic species of Campylobacter (C. coli and C. jejuni). When the chromosomal DNAs prepared from the three Campylobacter species were digested with the restriction enzyme ApaI, electrophoretic profiles of the undigested chromosomal DNAs also demonstrated PFGE to be useful for that discrimination.