+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Purification and characterization of cytoplasmic 5',(3')-nucleotidase from rabbit spleen: Characteristic differences of the enzyme from the rat spleen nucleotidase



Purification and characterization of cytoplasmic 5',(3')-nucleotidase from rabbit spleen: Characteristic differences of the enzyme from the rat spleen nucleotidase



Comparative Biochemistry and Physiology B Comparative Biochemistry 103(2): 399-405



1. A 5'(3')-nucleotidase, which preferably hydrolyzed 3'-dTMP and 3'-dUMP, was highly purified from rabbit spleen cytosol. 2. The enzyme also hydrolyzes 3'-UMP, 5'-dUMP and guanine nucleotides, but does not hydrolyze any adenine nucleotides or cytosine nucleotides. 3. The activity is dependent upon Mg2+, Co2+ or Mn2+; the addition of deoxyinosine stimulates the activity, and the pH optimum for the hydrolysis of 3'-dTMP is 7.0. 4. Although the catalytic properties of the enzyme are similar to the 5'(3')-nucleotidase from rat spleen, these nucleotidases differ in their molecular disposition. 5. The charge state of the rabbit enzyme is slightly basic, and the subunit M(r) is about 27 kDa, while the value of the rat enzyme is 26 kDa. 6. Immunochemical experiments with the use of antibodies against the purified nucleotidase indicate that enzymes from rabbit spleen and from rat spleen are composed of different polypeptides.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009277181

Download citation: RISBibTeXText

PMID: 1330428

DOI: 10.1016/0305-0491(92)90311-e


Related references

Rapid Internalization of Plasma-Membrane 5-Nucleotidase in Rat Spleen Lymphocytes in Response to Rabbit Anti-(Rat Liver 5-Nucleotidase) Serum. Biochemical Society Transactions 7(5): 1023-1024, 1979

Rapid internalization of plasma-membrane 5'-nucleotidase in rat spleen lymphocytes in response to rabbit anti-(rat liver 5'-nucleotidase) serum. Biochemical Society Transactions 7(5): 1023-1024, 1979

Rat spleen cytoplasmic nucleotidase characterization and its physiological significance. International Journal of Biochemistry 20(12): 1405-1410, 1988

A 3'-deoxyribonucleotidase isolated from rabbit spleen cytosol differs from the rat spleen nucleotidase. Nucleic Acids Symposium Series 1990(22): 85-86, 1990

Solubilization and purification of the diphosphopyridine nucleotidase from beef spleen. Journal of Biological Chemistry 219(2): 823-832, 1956

Partial purification and properties of an acid nucleotidase from the postmicrosomal supernatant of rat spleen. Biochemical Journal 169(3): 597-605, 1978

Deoxyinosine-activated nucleotidase purified from rat spleen. Evidence for its identity with liver enzyme. Acta Chemica Scandinavica. Series A: Physical and Inorganic Chemistry B33(5): 384-390, 1979

Deoxy inosine activated nucleotidase ec 3.1.3.31 purified from rat spleen evidence for its identity with liver enzyme. Acta Chemica Scandinavica Series B Organic Chemistry & Biochemistry 33(5): 384-386, 1979

The role of the intracellular inhibitor of periplasmic UDP-sugar hydrolase (5'-nucleotidase) in Escherichia coli: cytoplasmic localisation of 5'-nucleotidase is conditionally lethal. Journal of Basic Microbiology 41(6): 329-337, 2001

Chicken erythrocyte pyrimidine 5'-nucleotidase: purification and characterization of the subclass I enzyme. Biological Chemistry 382(6): 919-924, 2001

Research on streptococcal diphosphopyridine nucleotidase. I. Partial purification and characterization of the enzyme. Zeitschrift für Hygiene und Infektionskrankheiten; Medizinische Mikrobiologie Immunologie und Virologie 147: 350-356, 1961

Purification of membrane 5' nucleotidase preparation and properties of anti 5' nucleotidase serum. Peeters, H (Ed ) Protides Of The Biological Fluids Proceedings Of The Colloquium, Vol 29 Xxvii+984p Pergamon Press: Oxford, England; Elmsford, N Y , Usa Illus P147-150, 1982

Nucleotidase activities in the soluble fraction of rat liver homogenate. Partial purification and properties of a 5'-nucleotidase with pH optimum 6.3. Biochimica et Biophysica Acta 178(3): 534-541, 1969

Studies on 5'- nucleotidase on bonito muscle - l. lsolation and purification of 5'- nucleotidase. Bulletin Jap Soc Scient Fish 3912: 1271-1278, 1973

Cooperativity of the concanavalin A inhibition of bovine milk fat globule membrane 5'-nucleotidase. Response to extraction of nucleotidase and of putative cytoplasmic surface coat components. Biochimica et Biophysica Acta 611(2): 333-341, 1980