EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Purification and partial characterization of chymotrypsin-like proteases from the digestive gland of the scallop Pecten maximus


Purification and partial characterization of chymotrypsin-like proteases from the digestive gland of the scallop Pecten maximus



Comparative Biochemistry and Physiology B Comparative Biochemistry and Molecular Biology 110(4): 777-784



DOI: 10.1016/0305-0491(94)00211-c

Three variants of a chymotrypsin-like protease were purified from scallop digestive glands successively by ion-exchange, gel filtration and high-performance liquid chromatographies. Enzyme activity was detected using succinyl-Ala-Ala-Pro-Phe-p-nitroanilide as a specific synthetic substrate for chymotrypsin. This proteinase was inhibited by chymostatin, diisopropylfluorophosphate and phenylmethylsulfonyl fluoride. Estimated molecular mass of the purified enzyme is around 32 kDa. These isoenzymes exhibit very low activities in hydrolyzing small synthetic specific substrates used for trypsic, elastolytic and collagenolytic measurements and referred mainly to a chymotrypsin-like proteinase. Very few differences were measured concerning pH profiles among the three isoenzymes. Stability is higher at low temperature for two variants. An N-terminal analysis was performed on one variant (B) among the three isoenzymes. The alignment of the N-terminal amino acid sequence indicates some homologies with abalone chymotrypsin-like protein and arthropod chymotrypsin proteases as well as with vertebrate serine protease counterparts (trypsin, chymotrypsin and elastase).

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009278554

Download citation: RISBibTeXText

Related references

Cadmium binding components of scallop pecten maximus digestive gland partial purification and characterization. Comparative Biochemistry and Physiology C Pharmacology Toxicology and Endocrinology 85(2): 259-268, 1986

Cadmium binding components of scallop (Pecten maximus) digestive gland, partial purification & characterization. Comparative Biochemistry and Physiology C Comparative Pharmacology 852: 259-268, 1986

Functional micro anatomy of the digestive gland of the scallop pecten maximus l. Aquatic Living Resources 4(3): 191-202, 1991

Seasonal digestive gland dynamics of the scallop Pecten maximus in the Bay of Brest (France). Journal of the Marine Biological Association of the United Kingdom 81(4): 663-671, 2001

Apidogenesis in the digestive gland of the bivalve mollusc Pecten maximus I Micro-anatomy of the digestive gland and structural modifications during an annual cycle Lapidogenese dans la glande digestive du mollusque bivalve Pecten maximus I Micro-anatomie de la glande digestive et modifications structurales au cours dun cycle annuel. Bulletin de la Societe Zoologique de France, 1281-2: 117-129, 2003

Distribution and linkage of domoic acid (amnesic shellfish poisoning toxins) in subcellular fractions of the digestive gland of the scallop Pecten maximus. Toxicon: Official Journal of the International Society on Toxinology 55(2-3): 606-611, 2010

Apidogenesis within the digestive gland of the bivalve mollusc Pecten maximus II Molecular implications Lapidogenese dans la glande digestive du mollusque bivalve Pecten maximus II Implications moleculaires. Bulletin de la Societe Zoologique de France, 1281-2: 131-140, 2003

Adipogenesis in the digestive gland of the bivalve mollusc Pecten maximus. I. Micro-anatomy of the digestive gland and structural modifications during an annual cycle. Bulletin de la Societe Zoologique de France 128(1/2): 117-129, 2003

Morphological and biochemical characterizations of the great scallop Pecten maximus metamorphosis Caracterisations morphologique et biochimique de la metamorphose de la coquille Saint-Jacques, Pecten maximus Lamarck. Comptes Rendus de l'Academie des Sciences Serie III Sciences de la Vie. Octobre; 32210: 847-853, 1999

Amylase on Pecten maximus (Mollusca, bivalves): protein and cDNA characterization; quantification of the expression in the digestive gland. Molecular Marine Biology and Biotechnology 6(3): 228-237, 1997

Demonstration of nutrient pathway from the digestive system to oocytes in the gonad intestinal loop of the scallop Pecten maximus L. Biological Bulletin 205(1): 83-92, 2003

Purification and properties of two molecular forms of arginine kinase from the adductor muscle of the scallop, Pecten maximus. Comparative Biochemistry and Physiology. B Comparative Biochemistry 99(2): 387-394, 1991

Metals and metalloids in the digestive gland of a bivalve pecten maximus intra cellular localization by electron probe micro analysis involvement of digestive mechanisms. Comptes Rendus des Seances de l'Academie des Sciences Serie III Sciences de la Vie 294(13): 673-678, 1982

Previously unknown apicomplexan species infecting Iceland scallop, Chlamys islandica (Müller, 1776), queen scallop, Aequipecten opercularis L., and king scallop, Pecten maximus L. Journal of Invertebrate Pathology 108(3): 147-155, 2011

Scallop Pecten maximus population dynamics in the Bay of Brest was yesterday different from today? Dynamique de la population de coquilles Saint-Jacques Pecten maximus de la rade de Brest hier etait-il different daujourdhui?. Annales de l'Institut Oceanographique 73(1): 89-100, 1998