+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Purification and properties of Saccharomyces cerevisiae cystathionine beta-synthase

Purification and properties of Saccharomyces cerevisiae cystathionine beta-synthase

Yeast 10(3): 333-339

ISSN/ISBN: 0749-503X

PMID: 8017103

DOI: 10.1002/yea.320100306

Cystathionine beta-synthase (beta-CTSase), which catalyses cystathionine synthesis from serine and homocysteine, was purified to homogeneity from Saccharomyces cerevisiae. The molecular mass of the enzyme was estimated to be 235 kDa by gel filtration and 55 kDa by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, indicating that it is a homotetramer. The N-terminal amino acid sequence of the enzyme perfectly coincided with that deduced from the nucleotide sequence of CYS4, except for the absence of initiation methionine. The purified beta-CTSase catalysed cysteine synthesis from serine (or O-acetylserine) and H-2S. From this finding, we discuss the multifunctional nature and evolutionary divergence of S-metabolizing enzymes.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 009278701

Download citation: RISBibTeXText

Related references

Purification and properties of Saccharomyces cerevisiae cystathionine β-synthase. Yeast (Chichester) 10(3): 333-339, 1994

Transsulfuration in Saccharomyces cerevisiae is not dependent on heme: purification and characterization of recombinant yeast cystathionine beta-synthase. Journal of Inorganic Biochemistry 81(3): 161-171, 2000

Interconversion of a pair of active-site residues in Escherichia coli cystathionine gamma-synthase, E. coli cystathionine beta-lyase, and Saccharomyces cerevisiae cystathionine gamma-lyase and development of tools for the investigation of their mechanisms and reaction specificity. Biochemistry and Cell Biology 87(2): 445-457, 2009

Purification, crystallization and preliminary crystallographic analysis of the catalytic core of cystathionine β-synthase from Saccharomyces cerevisiae. Acta Crystallographica. Section F Structural Biology Communications 70(Pt 3): 320-325, 2014

Cysteine biosynthesis in Saccharomyces cerevisiae: mutation that confers cystathionine beta-synthase deficiency. Journal of Bacteriology 170(12): 5883-5889, 1988

Functional rescue of mutant human cystathionine beta-synthase by manipulation of Hsp26 and Hsp70 levels in Saccharomyces cerevisiae. Journal of Biological Chemistry 284(7): 4238-4245, 2009

Cystathionine beta-synthase is coordinately regulated with proliferation through a redox-sensitive mechanism in cultured human cells and Saccharomyces cerevisiae. Journal of Cellular Physiology 192(1): 81-92, 2002

Cystathionine gamma-lyase of Saccharomyces cerevisiae: structural gene and cystathionine gamma-synthase activity. Yeast 9(4): 389-397, 1993

Purification and properties of cystathionine beta-synthase from human liver. Evidence for identical subunits. Journal of Biological Chemistry 253(18): 6523-6528, 1978

Purification and properties of phosphatidyl inositol synthase from the membranes of saccharomyces cerevisiae. Abstracts of the Annual Meeting of the American Society for Microbiology 83: K91, 1983

Purification and properties of tryptophan synthase from baker's yeast (Saccharomyces cerevisiae). Biochemical Journal 209(1): 151-157, 1983

Purification and properties of the arginine specific carbamoyl phosphate synthase ec from saccharomyces cerevisiae. Journal of General Microbiology 106(1): 145-152, 1978

Purification and properties of the arginine-specific carbamoyl-phosphate synthase from Saccharomyces cerevisiae. Journal of General Microbiology 106(1): 145-151, 1978

Purification and properties of Saccharomyces cerevisiae acetolactate synthase from recombinant Escherichia coli. European Journal of Biochemistry 185(2): 433-439, 1989

Purification, properties, and crystallization of Saccharomyces cerevisiae dihydropterin pyrophosphokinase-dihydropteroate synthase. Protein Expression and Purification 41(2): 355-362, 2005