Purple non-sulphur bacterium isolated from ancient cryogenic rocks of the Kolyma lowland

Burashnikova, E.N.; Gogotov, I.N.

Mikrobiologiya 63(5): 868-875


ISSN/ISBN: 0026-3656
Accession: 009281090

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The purple non-sulphur bacterium of the Rhodopseudomonas genus was isolated from ancient cryogenic rocks of the Kolyma lowland. The bacterium developed under both photoheterotrophic conditions in the presence of acetate, malate, lactate, succinate or formiate, and photoautotrophic ones using the H-2 as an electron donor. The weak growth was observed in dark under microaerobic heterotrophic conditions. The bacterium had bacteriochlorophyll "a" and carotenoids of the spirilloxanthene group-2. The optimal conditions for growth were at pH 6.5-7.0, t = +25 degree C and light intensity 5 Wt/m-2. The aminoacid composition was characterized by a high content of arginine, glutamic and asparagic acids. The cells grown under nitrogen deficiency exhibited a nitrogenase activity. The hydrogenase activity was low (0.5 nmol/min.mg protein) and was only observed in cells grown under photomixotrophic conditions. By its guanine and cytosine quantity in DNA (65.1-67.3%) and its genetic affinity, the bacterium isolated is mostly alose to Rhodopseudomonas palustris.