+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Quantitative analysis of cholesterol and cholesteryl esters in human atherosclerotic plaques using near-infrared Raman spectroscopy



Quantitative analysis of cholesterol and cholesteryl esters in human atherosclerotic plaques using near-infrared Raman spectroscopy



Atherosclerosis 140(1): 81-88, Sept



Raman spectroscopy is a non-destructive analytical technique and previous results have shown that qualitative analysis of the lipid component of human atheromatous arteries is feasible. In this paper, we describe a quantitative analytical method for cholesterol and cholesteryl esters in human atherosclerotic plaques, combined with Raman spectroscopic results, using partial least-squares (PLS) regression, a statistical multivariate method based on factorial analysis. Twenty-nine human atherosclerotic pooled samples were studied and the results of Raman spectroscopy coupled with the PLS method were compared to biochemical results. The standard error of prediction was 16.1, 13.6, 1.9, 3.3 and 3.4 mg/g for total cholesterol, free cholesterol, palmitate cholesteryl, oleate cholesteryl and linoleate cholesteryl, respectively. The repeatability of Raman spectroscopy was found to be excellent. Our results show that Raman spectroscopy is a promising technique to obtain a consistent and non-destructive quantitative analysis of cholesterol and cholesteryl esters in human atherosclerotic lesions. In situ and in vivo analysis is a possibility in the near future.

(PDF emailed within 0-6 h: $19.90)

Accession: 009286018

Download citation: RISBibTeXText

PMID: 9733218

DOI: 10.1016/s0021-9150(98)00119-1


Related references

Two-dimensional visualization of cholesterol and cholesteryl esters within human coronary plaques by near-infrared fluorescence angioscopy. Clinical Cardiology 33(12): 775-782, 2011

Use of near-infrared Raman spectroscopy for identification of atherosclerotic plaques in the carotid artery. Photomedicine and Laser Surgery 25(6): 482-486, 2007

Determination of cholesterol in atherosclerotic plaques using near infrared diffuse reflection spectroscopy. Atherosclerosis 147(2): 327-337, 1999

Quantification of cholesteryl esters in human and rabbit atherosclerotic plaques by magic-angle spinning (13)C-NMR. Arteriosclerosis, Thrombosis, and Vascular Biology 20(12): 2682-2688, 2000

Proton nmr spectroscopy of cholesteryl esters in human atherosclerotic plaque. Biophysical Journal 51(2 PART 2): 78A, 1987

Determination of the cholesterol-collagen ratio of arterial atherosclerotic plaques using near infrared spectroscopy as a possible measure of plaque stability. Atherosclerosis 165(2): 251-257, December, 2002

Determination of the cholesterol-collagen-ratio of atherosclerotic arterial plaques using near infrared spectroscopy as a possible measure of plaque vulnerability. Clinical Chemistry 48(6 Supplement): A105, June, 2002

The composition of cholesterol esters in fatty streaks and atherosclerotic plaques of the human aorta. Histochromatographic investigations. Atherosclerosis 13(1): 93-101, 1971

Quantitative analysis of the content of deltamethrin in agrochemicals by near-infrared, attenuated total reflectance infrared and raman spectroscopy. Guang Pu Xue Yu Guang Pu Fen Xi 30(11): 2936-2940, 2011

Quantitative analysis of sulfathiazole polymorphs in ternary mixtures by attenuated total reflectance infrared, near-infrared and Raman spectroscopy. Journal of Pharmaceutical and Biomedical Analysis 53(3): 412-420, 2010

Detection of lipid pool, thin fibrous cap, and inflammatory cells in human aortic atherosclerotic plaques by near-infrared spectroscopy. Circulation 105(8): 923-927, February 26, 2002

Stimulation of cholesteryl ester synthesis in macrophages by extracts of atherosclerotic human aortas and complexes of albumin/cholesteryl esters. Arteriosclerosis 1(3): 210-226, 1981

Plasma cholesteryl esters provided by lecithin:cholesterol acyltransferase and acyl-coenzyme a:cholesterol acyltransferase 2 have opposite atherosclerotic potential. Circulation Research 95(10): 998-1004, 2004