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Quantitative determination of cucurbitacins by high performance liquid chromatography and high performance thin layer chromatography


Quantitative determination of cucurbitacins by high performance liquid chromatography and high performance thin layer chromatography



Journal of Liquid Chromatography 16(2): 497-511



ISSN/ISBN: 0148-3919

DOI: 10.1080/10826079308020927

A simple and precise method using High-Performance Liquid (HPLC) and High-Performance Thin-Layer Chromatography (HPTLC) was developed for simultaneous determination of the frequently encountered cucurbitacins B (1), D (2), 3-epi-iso-cucurbitacin D (3), E (4) and I (5) as well as cucurbitacin glycosides E (6) and I (7). A reverse phase column (C-18) was used and elution achieved with acetonitrile-water (2:8, solvent A and 45:55, solvent B) with gradient elution analysis. Reverse-phase HPTLC plates were developed with methanol:water (7:3) and normal-phase plates were developed with toluene-ethylacetate (25:75). Developed plates were scanned with Thin-Layer Scanning Instrumentation. A clean-up procedure was perfected before sample loading to both the HPLC and HPTLC.

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Accession: 009287274

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