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Quantitative determination of tannin and protein in the precipitates by high-performance liquid chromatography

Quantitative determination of tannin and protein in the precipitates by high-performance liquid chromatography

Phytochemistry (Oxford) 40(5): 1503-1505

ISSN/ISBN: 0031-9422

DOI: 10.1016/0031-9422(95)00451-c

High performance liquid chromatography was applied for the determination of both tannin and protein in tannin-protein coprecipitates and the relative affinities of a series of galloylglucoses for bovine serum albumin were determined. Relative affinity is affected mainly by the number of the galloyl groups in the galloylglucose molecule with penta gt tetra- gt tri- mchgt di- mchgt monogalloylglucose. The position of the galloyl group in the glucose core also affects the relative affinity but the effect is smaller than that of the number with 2,3,6- gt 2,3,4-tri-O-galloylglucose and 4,6- gt 2,3-di-O-galloylglucose.

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Accession: 009287387

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