Random amplified polymorphic DNA and restriction fragment length polymorphism analyses of Cannabis sativa
Shirota, O.; Watanabe, A.; Yamazaki, M.; Saito, K.; Shibano, K.; Sekita, S.; Satake, M.
Natural Medicines 52(2): 160-166
Random Amplified Polymorphic DNA (RAPD) and Restriction Fragment Length Polymorphism (RFLP) methods were used for the analysis of three different strains of Cannabis sativa that included the Mexican (drug type), Tochigishiro (fiber type) and Nara cultivated strains. Cannabis saliva samples were grouped into three chemotypes, drug type, fiber type and intermediate drug type by GC analysis. The RAPD analysis was able to distinguish the three chemotypes clearly, however, in each type, some genetic diversities was shown. The RFLP analysis showed that the genetic distance between the Tochigishiro and Nara cultivated strains was small, whereas that between the Mexican strain and the other two strains was large, and that there was no genetic diversity within each strain. These results indicated that the RAPD and RFLP analyses, using DNA as a marker, applied in combination might be useful for distinguishing between the strains having chemotypes.