EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Rapid high-performance liquid chromatographic quantification of recombinant human antithrombin III during production and purification


Rapid high-performance liquid chromatographic quantification of recombinant human antithrombin III during production and purification



Journal of Chromatography. B Biomedical Applications 662(2): 209-216



ISSN/ISBN: 1572-6495

PMID: 7719477

DOI: 10.1016/0378-4347(94)00266-5

For monitoring of recombinant human antithrombin III during cell culture processes and subsequent purification steps a rapid method for quantitative determination was developed. The need for the introduction of this rapid method came from the limited availability of a quantitative enzyme-linked immunosorbent assay (ELISA) and the very time-consuming ELISA procedure. The developed method is based on reversed-phase high-performance liquid chromatography using a C-4 column. The separation by gradient elution using water and acetonitrile takes less than 20 min even when complex samples, such as serum containing cell culture samples, have to be analyzed. Automation and a high sample throughput are possible with this reliable method. If necessary, insulin, transferrin and albumin can also be quantified with minor changes of the elution profile.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009298365

Download citation: RISBibTeXText

Related references

Rapid, sensitive high-performance liquid chromatographic method for the quantification of promethazine in human serum with electrochemical detection. Journal of Chromatography 631(1-2): 255-259, 1993

Rapid isocratic high-performance liquid chromatographic purification of platelet activating factor (PAF) and lyso-PAF from human skin. Journal of Chromatography 309(1): 160-164, 1984

Simple and rapid high-performance liquid chromatographic method for the quantification of 3-methylhistidine. Journal of Chromatography 223(2): 417-420, 1981

Rapid high-performance liquid chromatographic method for the quantification of a-tomatine in tomato. Journal of Agricultural and Food Chemistry 42: 75-7, 1994

An isocratic high performance liquid chromatographic method for quantification of mycophenolic acid and its glucuronide metabolite in human serum using liquid-liquid extraction: application to human pharmacokinetic studies. Clinica Chimica Acta; International Journal of Clinical Chemistry 370(1-2): 185-190, 2006

Rapid and inexpensive high-performance liquid chromatographic method for the quantification of flecainide in serum. Journal of Chromatography 427(2): 351-358, 1988

Rapid high-performance liquid chromatographic method for the quantification of alpha-tomatine in tomato. Journal of Agricultural and Food Chemistry 42(11): 2475-2477, 1994

Rapid high-performance liquid chromatographic method for the quantification of mexiletine and its metabolites in serum. Journal of Chromatography 493(2): 414-420, 1989

Determination of chlorophyll in marine waters intercomparison of a rapid high performance liquid chromatographic method with full high performance liquid chromatographic spectrophotometric and fluorometric methods. Marine Chemistry 19(3): 211-228, 1986

Simple and rapid method for high-performance liquid chromatographic separation and quantification of soybean phospholipids. Journal of Chromatography 447(2): 436-442, 1988

High-performance liquid chromatographic purification and capillary electrophoresis quantification of the chemokine stromal cell-derived factor-1. Journal of Chromatography. B Biomedical Sciences and Applications 729(1-2): 369-374, 1999

Reversed-phase high-performance liquid chromatographic system for the rapid, automated purification of oligonucleotides. Journal of Chromatography 402: 381-386, 1987

Extraction, pre-high-performance liquid chromatographic purification, and high-performance liquid chromatographic analysis of plant nucleotides. Analytical Biochemistry 183(2): 312-319, 1989

Rapid and sensitive on-line precolumn purification and high-performance liquid chromatographic assay for disulfiram and its metabolites. Journal of Chromatography 378(2): 419-429, 1986

High-performance liquid chromatographic system for the rapid purification of growth-hormone receptor in rabbit livers. Advances in Chromatography 1986. International Symposium 22. Advances in Chromatography 1986. International Symposium 23 (Houston, 1986) 400: 279-283, 1987