+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Recombination during transformation as a source of chimeric mammalian artificial chromosomes in yeast (YACs)



Recombination during transformation as a source of chimeric mammalian artificial chromosomes in yeast (YACs)



Nucleic Acids Research 22(20): 4154-4162



Mammalian DNAs cloned as artificial chromosomes in yeast (YACs) frequently are chimeras formed between noncontiguous DNAs. Using pairs of human and mouse YACs we examined the contribution of recombination during transformation or subsequent mitotic growth to chimeric YAC formation. The DNA from pairs of yeast strains containing homologous or heterologous YACs was transformed into a third strain under conditions typical for the development of YAC libraries.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009307727

Download citation: RISBibTeXText

PMID: 7937141

DOI: 10.1093/nar/22.20.4154


Related references

Isolation and analysis of large contiguous regions of mammalian chromosomes in yeast artificial chromosomes yacs. American Journal of Human Genetics 49(4 Suppl.): 10, 1991

Targeted integration of neomycin into yeast artificial chromosomes (YACs) for transfection into mammalian cells. Nucleic Acids Research 20(12): 2971-2976, 1992

Use of yeast artificial chromosomes (YACs) in studies of mammalian development: production of b-globin locus YAC mice carrying human globin developmental mutants. Proceedings of the National Academy of Sciences of the United States of America 92: 55-9, 1995

Use of yeast artificial chromosomes (YACs) in studies of mammalian development: production of beta-globin locus YAC mice carrying human globin developmental mutants. Proceedings of the National Academy of Sciences of the United States of America 92(12): 5655-5659, 1995

Physical mapping of yeast artificial chromosomes yacs on human chromosomes 21 and 18 by fluorescence in situ hybridization. American Journal of Human Genetics 49(4 Suppl.): 382, 1991

Rapid cloning of mouse DNA as yeast artificial chromosomes by transformation-associated recombination (TAR). Mammalian Genome 9(2): 157-159, 1998

Specific cloning of human DNA as yeast artificial chromosomes by transformation-associated recombination. Proceedings of the National Academy of Sciences of the United States of America 93(1): 491-496, 1996

Cloning of the hla complex in yeast artificial chromosomes yacs. Complement & Inflammation 8(3-4): 135, 1991

Isolation of yeast artificial chromosomes (YACs) of porcine chromosome. 1999

Yeast artificial chromosomes (YACs) and the analysis of complex genomes. Trends in Biotechnology 10(1-2): 35-40, 1992

Isolation and characterization of yeast artificial chromosomes yacs from the hla complex. American Journal of Human Genetics 49(4 Suppl.): 375, 1991

The human immunoglobulin k locus on yeast artificial chromosomes (YACs). Gene 2 1997): 3-81, 1997

The human immunoglobulin kappa locus on yeast artificial chromosomes (YACs). Gene 191(2): 173-181, 1997

Physical mapping of rice chromosome 1 with yeast artificial chromosomes (YACs). Dna Research 3(5): 291-296, 1996

Topoisomerase 2 is dispensable for the replication and segregation of small yeast artificial chromosomes (YACs). Plos one 9(8): E104995, 2014