+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Reconstruction of the urinary bladder mucosa in three-dimensional collagen gel culture: Fibroblast-extracellular matrix interactions on the differentiation of transitional epithelial cells

Reconstruction of the urinary bladder mucosa in three-dimensional collagen gel culture: Fibroblast-extracellular matrix interactions on the differentiation of transitional epithelial cells

Journal of Urology 153(6): 2060-2067

The purpose of this study was to reconstruct a urinary bladder mucosa in three-dimensional collagen gel culture conditions that included fibroblasts. Transitional epithelial cells and fibroblasts, isolated respectively from the epithelial and lamina propria layers of porcine urinary bladder, were cultured in monolayer. These fibroblasts were embedded and cultured within a collagen gel matrix to reconstruct a lamina propria. The isolated transitional epithelial cells were then seeded in vitro on this reconstructed lamina propria on which the transitional epithelial cells formed a stratified urothelium composed of basal, intermediate and superficial layers. Urothelial differentiation was observed only on the fibroblast-containing collagen matrix. Differentiation did not occur on a cell-free collagen matrix through use of fibroblast conditioned medium. Thus, urothelial differentiation depended upon a fibroblast-extracellular matrix interaction. The differentiated transitional epithelial cell layer thus produced closely resembled a urothelium in vivo. This culture model may provide a useful system in which to study various diseases of the urinary bladder.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 009308636

Download citation: RISBibTeXText

PMID: 7752394

DOI: 10.1016/s0022-5347(01)67402-0

Related references

Reconstruction of alveolus-like structure from alveolar type II epithelial cells in three-dimensional collagen gel matrix culture. American Journal of Pathology 142(3): 783-792, 1993

Reconstruction of the laryngeal mucosa. A three-dimensional collagen gel matrix culture. Archives of Otolaryngology--Head & Neck Surgery 122(6): 649-654, 1996

Differentiation of human endometrial stem cells into urothelial cells on a three-dimensional nanofibrous silk-collagen scaffold: an autologous cell resource for reconstruction of the urinary bladder wall. Journal of Tissue Engineering and Regenerative Medicine 9(11): 1268-1276, 2015

Reconstruction of prostatic acinus-like structure from ventral and dorsolateral prostatic epithelial cells of the rat in three-dimensional collagen gel matrix culture. Journal of Urology 157(3): 1025-1031, 1997

Epithelial cell-fibroblast interactions: Modulation of extracellular matrix proteins in cultured oral cells. Journal of Periodontal Research 28(2): 102-114, 1993

Three-dimensional culture environment increases the efficacy of platelet rich plasma releasate in prompting skin fibroblast differentiation and extracellular matrix formation. International Journal of Medical Sciences 11(10): 1029-1038, 2014

An air-liquid interface promotes the differentiation of gastric surface mucous cells Reconstruction of the gastric surface epithelial layer in three-dimensional collagen gel culture. Gastroenterology 120(5 Suppl. 1): A 301, 2001

Collagen three-dimensional hydrogel matrix carrying basic fibroblast growth factor for the cultivation of mesenchymal stem cells and osteogenic differentiation. Tissue Engineering. Part a 18(9-10): 1087-1100, 2012

Extracellular Matrix-Dependent Differentiation of Rabbit Tracheal Epithelial Cells in Primary Culture. In Vitro Cellular & Developmental Biology. Animal 30A(1): 56-67, 1994

Extracellular matrix-dependent differentiation of rabbit tracheal epithelial cells in primary culture. In Vitro Cellular & Developmental Biology Animal. 30a(1): 56-67, 1994

Effects of growth factor and extracellular matrix on types of collagen synthesized by bovine lens epithelial cells in culture. Biology of the Cell 45(1): 21, 1982

Tubule formation and functional differentiation by human epithelial respiratory cells cultured in a three-dimensional collagen matrix. Chest 101(3 Suppl.): 7s-9s, 1992

Evaluation of extracellular matrix in non tumorous changes and transitional cell tumors of the urinary bladder. Jikeikai Medical Journal 37(2): 103-114, 1990

Influences of extracellular matrix components on the growth and differentiation of ruminal epithelial cells in primary culture. Research in Veterinary Science 54(1): 102-109, 1993

In vitro differentiation of germ cells from nonobstructive azoospermic patients using three-dimensional culture in a collagen gel matrix. Fertility and Sterility 87(4): 824-833, 2007