+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Redistribution of insoluble interphotoreceptor matrix components during photoreceptor differentiation in the mouse retina

Redistribution of insoluble interphotoreceptor matrix components during photoreceptor differentiation in the mouse retina

Journal of Comparative Neurology 345(1): 115-124

The development of the nervous system is largely influenced by the extracellular matrix (ECM). In the neural retina, the photoreceptors are surrounded by a unique ECM, the interphotoreceptor matrix (IPM). The IPM plays a central and possibly crucial role in the development, maintenance and specific function of the photoreceptors. Therefore, the characterization of IPM components is necessary to understand the mechanisms regulating photoreceptor differentiation. The IPM in the mouse retina was examined during photoreceptor morphogenesis with the monoclonal antibody (MAb) F22, which recognizes a 250 kDa component of the interphotoreceptor matrix. The binding pattern of MAb F22 revealed a striking redistribution in the expression of the 250 kDa F22 antigen in late stage of postnatal photoreceptor differentiation in the mouse retina. The F22 staining was detectable in the IPM around the inner segments on the third postnatal day (P3). The MAb F22 initially labeled the region around inner segments, but as the outer segments elongated, the F22 distribution became concentrated to the matrix around the rod and cone outer segments until P16-17. At P17, the F22 label around rods began to disappear, while the label around cones became more defined. The shift in label distribution was largely completed by P20. Residual rod-associated label disappeared within a few days. In the adult animal, the F22 antibody labeled the cone-associated matrix only, and this labeling pattern remained stationary. The change in the distribution of MAb F22 demonstrated by immunolabeling was not accompanied by changes in the size of the molecule; F22 antigen isolated from the IPM of P13-15, and from adult IPM migrated with the same molecular weight on SDS gels. The distribution of MAb F22 was compared to that of chondroitin sulfate proteoglycans which are abundant in the IPM. The labeling patterns of MAbs CS-56, C6-S and C4-S were distinct from that of MAb F22. A general decrease of the label intensity was seen with two chondroitin sulfate MAbs (CS-56 and C4-S) between 16 days and 4 months, but a total loss of rod-associated label was not observed. All three chondroitin sulfate MAbs labeled the retina at embryonic day (E) 11.5-13.5, a time of outgrowth of ganglion cell axons, but the F22 antigen was not detected in the retina at this stage of development. The results demonstrate that the F22 and the chondroitin sulfate antibodies are recognizing different molecules that have distinct roles in retinal morphogenesis.(ABSTRACT TRUNCATED AT 400 WORDS)

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 009311757

Download citation: RISBibTeXText

PMID: 8089273

DOI: 10.1002/cne.903450109

Related references

Development and fate of interphotoreceptor matrix components during dysplastic photoreceptor differentiation: a lectin cytochemical study of rod-cone dysplasia 1. Experimental Eye Research 56(4): 429-441, 1993

Early expression of the gene for interphotoreceptor retinol-binding protein during photoreceptor differentiation suggests a critical role for the interphotoreceptor matrix in retinal development. Journal of Cell Biology 111(6 Pt 1): 2775-2784, 1990

Insoluble interphotoreceptor matrix domains surround rod photoreceptors in the human retina. Experimental Eye Research 51(1): 107-110, 1990

Is photoreceptor-RPE binding enhanced by components of the interphotoreceptor matrix?. Investigative Ophthalmology & Visual Science 36(4): S510, 1995

Photoreceptor synthesis secretion and retrieval of components in the interphotoreceptor matrix. Journal of Cell Biology 99(4 Part 2): 115A, 1984

Participation of photoreceptor cells in retrieval and degradation of components in the interphotoreceptor matrix. Progress in Clinical and Biological Research 190: 171-175, 1985

Interphotoreceptor matrix-poly(ϵ-caprolactone) composite scaffolds for human photoreceptor differentiation. Journal of Tissue Engineering 5: 2041731414554139, 2014

Interactions of the interphotoreceptor retinol binding protein with proteins of the insoluble interphotoreceptor matrix. Investigative Ophthalmology & Visual Science 38(4 Part 1-2): S3, 1997

Proteoglycans in the mouse interphotoreceptor matrix. III. Changes during photoreceptor development and degeneration in the rds mutant. Experimental Eye Research 51(3): 301-315, 1990

Photoreceptor degeneration and altered distribution of interphotoreceptor matrix proteoglycans in the mucopolysaccharidosis VII mouse. Experimental Eye Research 56(5): 531-541, 1993

Proteoglycans in the mouse interphotoreceptor matrix: VI. Evidence for photoreceptor synthesis of chondroitin sulfate proteoglycan using genetically fractionated retinas. Experimental Eye Research 55(2): 345-356, 1992

The interphotoreceptor matrix and the interphotoreceptor space of the vertebrate retina. Scanning Electron Microscopy 1985(Pt 2): 859-868, 1985

SPACR in the interphotoreceptor matrix of the mouse retina: molecular, biochemical and immunohistochemical characterization. Experimental Eye Research 71(4): 341-352, 2000