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Regulation of activating transcription factor-1 and the cAMP response element-binding protein by Ca2+/calmodulin-dependent protein kinases type I, II, and IV

Sun, P.; Lou, L.; Maurer, R.A.

Journal of Biological Chemistry 271(6): 3066-3073

1996


ISSN/ISBN: 0021-9258
PMID: 8621702
Accession: 009320313

The ability of activating transcription factor-1 (ATF1) or the cAMP response element-binding protein (CREB) to enhance transcription can be stimulated by increases in intracellular Ca-2+ concentrations. To identify protein kinases which may mediate the ability of Ca-2+ to activate these transcription factors, we compared the ability of constitutively active forms of several Ca-2+/calmodulin-dependent protein kinases (CaM kinases) to activate ATF1 or CREB. We find that constitutively active CaM kinase I and IV can activate both ATF1 and CREB. In addition, expression vectors for full-length CaM kinase I and IV were able to augment the ability of Ca-2+ influx to activate ATF1 or CREB consistent with a role for these kinases in mediating transcriptional responses to Ca-2+ signaling. In contrast, CaM kinase II was unable to activate either ATF1 or CREB. These findings provide a potential mechanism that may permit variation in the ability of ATF1 and CREB to respond to changes in intracellular Ca-2+ concentrations depending on differences in the relative concentrations of specific CaM kinases.

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