+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Regulation of c-fos expression by RNA polymerase elongation competence



Regulation of c-fos expression by RNA polymerase elongation competence



Journal of Molecular Biology 280(5): 785-798



The molecular mechanisms underlying transcription elongation and their role in gene regulation are poorly characterized in eukaryotes. A number of genes, however, have been proposed to be regulated at the level of transcription elongation, including c-myc, c-fos and c-myb. Here, we analyze the control of transcription elongation at the mouse c-fos gene at the nucleotide level in intact cells. We find that RNA polymerases are engaged in the promoter-proximal part of the gene in the absence of gene activation signals and mRNA synthesis. Importantly, we determine that the engaged RNA polymerases originate from a continuous initiation of transcription which, in the absence of gene activation signals, terminate close to the promoter. We also observe that the c-fos gene presents an active chromatin conformation, with the promoter and upstream regulatory sequences constitutively occupied by proteins, accounting for the continuous initiation of RNA polymerase complexes. We propose that activation of c-fos gene expression results primarily from the assembly of elongation-competent RNA polymerases that can transcribe the complete gene. Our results suggest that the engaged RNA polymerases found downstream of a number of other eukaryotic promoters may be associated with transcription termination of elongation-incompetent polymerases in the absence of activating signals.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009320764

Download citation: RISBibTeXText

PMID: 9671550

DOI: 10.1006/jmbi.1998.1905


Related references

Regulation of heat shock gene expression by RNA polymerase II elongation factor, Elongin A. Journal of Biological Chemistry 280(6): 4017-4020, 2005

Negative elongation factor-mediated suppression of RNA polymerase II elongation of Kaposi's sarcoma-associated herpesvirus lytic gene expression. Journal of Virology 86(18): 9696-9707, 2012

The RNA polymerase-associated factor 1 complex (Paf1C) directly increases the elongation rate of RNA polymerase I and is required for efficient regulation of rRNA synthesis. Journal of Biological Chemistry 285(19): 14152-9, 2010

RNA polymerase II elongation factors Spt4p and Spt5p play roles in transcription elongation by RNA polymerase I and rRNA processing. Proceedings of the National Academy of Sciences of the United States of America 103(34): 12707-12712, 2006

Structure and function of RNA polymerase II elongation factor ELL. Identification of two overlapping ELL functional domains that govern its interaction with polymerase and the ternary elongation complex. Journal of Biological Chemistry 272(35): 22355-22363, 1997

Regulation of RNA polymerase II elongation by c-Myc. Cell 141(3): 399-400, 2010

Regulation of competence for genetic transformation in Streptococcus pneumoniae: Expression of dpnA, a late competence gene encoding a DNA methyltransferase of the DpnII restriction system. Molecular Microbiology 35(5): 1089-1098, 2000

Regulation of transcriptional elongation by RNA polymerase II. Current Opinion in Genetics & Development 5(2): 210-216, 1995

RNA polymerase: regulation of transcript elongation and termination. Faseb Journal 5(13): 2833-2842, 1991

Mechanism and regulation of transcriptional elongation by RNA polymerase II. Current Opinion in Cell Biology 11(3): 342-346, 1999

Regulation of ribosomal RNA production by RNA polymerase I: does elongation come first?. Genetics Research International 2012: 276948, 2012

DSIF and NELF interact with RNA polymerase II elongation complex and HIV-1 Tat stimulates P-TEFb-mediated phosphorylation of RNA polymerase II and DSIF during transcription elongation. Journal of Biological Chemistry 276(16): 12951-8, 2001

The regulation of elongation by eukaryotic RNA polymerase II: a recent view. Molecules and Cells 11(3): 267-274, 2001

Mechanism and regulation of transcriptional elongation and termination by RNA polymerase II. Current Opinion in Genetics & Development 7(2): 199-204, 1997