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Repeated sequence RS-alpha is diagnostic for Bradyrhizobium japonicum and Bradyrhizobium elkanii



Repeated sequence RS-alpha is diagnostic for Bradyrhizobium japonicum and Bradyrhizobium elkanii



Biology & Fertility of Soils 23(1): 15-19



The genome of Bradyrhizobium japonicum and B. elkanii contains multiple copies of the repeated DNA sequence RS-alpha. A collection of 18 B. japonicum, 4 B. elkanii and 72 other bacterial strains was screened by polymerase chain reaction (PCR) using a pair of primers specific for RS-alpha. Only strains of B. japonicum and B. elkanii gave the predicted amplification product. Restriction analysis of PCR products obtained from different strains of B. japonicum showed that the RS-alpha sequence was generally conserved. The usefulness of RS-alpha as a specific probe for Bradyrhizobium strains capable of nodulating soybean was also demonstrated.

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