EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Resonance Raman spectroscopy of the B820 subunit of the core antenna from Rhodospirillum rubrum G9



Resonance Raman spectroscopy of the B820 subunit of the core antenna from Rhodospirillum rubrum G9



Biochimica et Biophysica Acta 1183(2): 369-373



The resonance Raman spectrum in the carbonyl-stretch region (1600-1700 cm-1) of the B820(OG) subunit form is completely different from the original core antenna complex of Rhodospirillum rubrum G9. The observed changes appear to be fully reversible upon reassociation of the B820(OG) subunits into the native-like B873(reassoc). This allows direct conclusions regarding the BChl a binding-site in the antenna complex. In the native complex all 2-acetyl carbonyl groups are hydrogen bonded since only a single resonance peak is observed at 1640 cm-1. When the core antenna is dissociated into its subunit B820(OG) form, half of this vibrational band is shifted to 1625 cm-1, indicating that approx. 50% of the 2-acetyl groups of the BChl a porphyrin rings have changed their hydrogen bond interaction. Besides these changes, there are also effects in the resonance frequency of the 9-keto carbonyl group that indicate that, upon dissociation, a fraction of these groups becomes free from interactions. Similar changes are observed for the B820(ODPS) subunit that is prepared using a different detergent. The spectral shifts upon dissociation cannot be attributed solely to the change in hydrogen bonding, suggesting that an additional perturbation of the BChl a binding-site takes place. Possible models for the hydrogen bonding of these groups are discussed.

(PDF emailed within 0-6 h: $19.90)

Accession: 009345758

Download citation: RISBibTeXText

DOI: 10.1016/0005-2728(93)90241-7



Related references

Fluorescence site selection spectroscopy of the B820 subunit from the core antenna from Rhodospirillum rubrum G9. Murata, N Research in photosynthesis, Vol 1; IXth International Congress on Photosynthesis: 37-40, 1992

Self assembly of the LH-1 antenna of Rhodospirillum rubrum A time-resolved study of the aggregation of the B820 subunit form. Murata, N Research in photosynthesis, Vol 1; IXth International Congress on Photosynthesis : 101-104, 1992

Exciton interactions in the light harvesting antenna of photosynthetic bacteria studied with triplet singlet spectroscopy and singlet triplet annihilation on the b820 subunit form of rhodospirillum rubrum. Biochimica et Biophysica Acta 1059(1): 111-119, 1991

Spectroscopic characterisation of a tetrameric subunit form of the core antenna protein from Rhodospirillum rubrum. FEBS Letters 528(1-3): 222-226, 25 September, 2002

Resonance Raman spectroscopy of cytochrome bc1 complexes from Rhodospirillum rubrum: initial characterization and reductive titrations. Biochimica et Biophysica Acta 1018(1): 47-54, 1990

Surface-enhanced resonance Raman scattering spectroscopy of bacterial photosynthetic membranes. The carotenoid of Rhodospirillum rubrum. Journal of Biological Chemistry 263(9): 4374-4380, 1988

The reaction order of the dissociation reaction of the B820 subunit of Rhodospirillum rubrum light-harvesting I complex. FEBS Letters 516(1-3): 40-42, 10 April, 2002

The effect of pressure on the bacteriochlorophyll a binding sites of the core antenna complex from Rhodospirillum rubrum. Biochemistry 37(42): 14875-14880, 1998

The effect of pressure on the bacteriochlorophyll alpha binding sites of the core antenna complex from Rhodospirillum rubrum. Biochemistry 37(42): 14875-14880, Oct 20, 1998

Inhibition of atp linked activities of rhodospirillum rubrum and of lettuce by antibodies raised against rhodospirillum rubrum f 1 atpase and its beta subunit. Israel Journal of Medical Sciences 17(6): 488, 1981

Antibodies to the f 1 atpase of rhodospirillum rubrum and its purified native beta subunit inhibition of atp linked activities in rhodospirillum rubrum and in lettuce. European Journal of Biochemistry 119(1): 107-114, 1981

Structure of S0 lycopene in antenna complex of Rhodospirillum molischianum DSM 119 as revealed by Raman spectroscopy. Plant & Cell Physiology 39(SUPPL ): S28, 1998

Intermolecular vibrational coherence in the bacteriochlorophyll proteins B777 and B820 from Rhodospirillum rubrum. Journal of Physical Chemistry. B 112(4): 1299-1307, 2008