EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Resonance Raman spectroscopy of the photosystem II light-harvesting complex of green plants: A comparison of trimeric and aggregated states



Resonance Raman spectroscopy of the photosystem II light-harvesting complex of green plants: A comparison of trimeric and aggregated states



Biochemistry 34(7): 2333-2337



Resonance Raman spectroscopy was performed on photosystem II light-harvesting complex (LHCII) in trimeric and oligomeric states with various excitation conditions. From these studies, it can be concluded that the structure of LHCII is altered during the trimer/oligomer transition. These structural changes affect the conformation of a population of carotenoid within the complex, which becomes twisted in the oligomeric form. Moreover, the interactions assumed by a chlorophyll a and a chlorophyll b are modified during the oligomerization process. This induces the formation of an H-bond to a formyl group of a chlorophyll b molecule and to a keto group of a chlorophyll a molecule. The extent to which these H-bonds to chlorophyll relate to the formation of the quencher cannot yet be precisely established. However, the structural changes they evidence may play a role in the control of the energy flux by LHCII complexes.

(PDF emailed within 0-6 h: $19.90)

Accession: 009345761

Download citation: RISBibTeXText

PMID: 7857944

DOI: 10.1021/bi00007a029



Related references

Resonance coherent anti stokes raman scattering of chlorophyll iv. coherent anti stokes raman spectroscopy spectrum of the light harvesting chlorophyll a b protein complex of photosystem ii from vicia faba after excitation at 694.3 nanometer. Studia Biophysica 113(3): 105-170, 1986

The functional significance of the monomeric and trimeric states of the photosystem II light harvesting complexes. Biochemistry 43(2): 501-509, 2004

Functional analyses of the plant photosystem I-light-harvesting complex II supercomplex reveal that light-harvesting complex II loosely bound to photosystem II is a very efficient antenna for photosystem I in state II. Plant Cell 24(7): 2963-2978, 2013

Acclimation to intense light implies changes at the level of trimeric subunits involved in the structural organization of the main light-harvesting complex of photosystem II (LHCII) and their isoforms. Plant Physiology and Biochemistry 50(1): 8-14, 2012

Three-dimensional reconstruction of a light-harvesting complex I-photosystem I (LHCI-PSI) supercomplex from the green alga Chlamydomonas reinhardtii. Insights into light harvesting for PSI. Journal of Biological Chemistry 278(18): 16135-16141, 2003

Heterogeneity of the main light-harvesting chlorophyll a/b-protein complex of photosystem II (LHCII) at the level of trimeric subunits. Journal of Photochemistry and Photobiology B Biology 64(1): 45-54, 2001

Configuration and dynamics of xanthophylls in light-harvesting antennae of higher plants. Spectroscopic analysis of isolated light-harvesting complex of photosystem II and thylakoid membranes. Journal of Biological Chemistry 276(27): 24862-24870, 2001

The acclimative response of the main light-harvesting chlorophyll a/b-protein complex of photosystem II (LHCII) to elevated irradiances at the level of trimeric subunits. Journal of Photochemistry and Photobiology B, Biology 70(3): 163-170, 2003

Low-temperature spectroscopy of monomeric and trimeric forms of reconstituted light-harvesting chlorophyll a/b complex. Biochimica Et Biophysica Acta. 1273(2): 171-174, 1996

Fluorescence emission spectra of photosystem I, photosystem II and the light-harvesting chlorophyll a/b complex of higher plants. Biochimica et Biophysica Acta 462(2): 307-313, 1977

Pigment organisation in the membrane-intrinsic major light-harvesting complex of Amphidinium carterae: Structural characterisation of the peridinins and chlorophylls a and c2 by resonance Raman spectroscopy and from sequence analysis. Biochimica et Biophysica Acta 1847(10): 1187-1199, 2015

Generation of fluorescence quenchers from the triplet states of chlorophylls in the major light-harvesting complex II from green plants. Biochemistry 39(34): 10468-10477, 2000