+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Retinoid regulation of human ectocervical epithelial cell transglutaminase activity and keratin gene expression



Retinoid regulation of human ectocervical epithelial cell transglutaminase activity and keratin gene expression



Differentiation 54(3): 219-225



Cornified envelope formation, the level of transglutaminase activity and the pattern of cytokeratin gene expression are important biochemical markers of cervical epithelial cell differentiation in vivo. In the present study we examine the effects of retinoid treatment on transglutaminase (TG) activity and keratin gene expression in cultured human ectocervical epithelial cells (ECE cells). All-trans-retinoic acid (RA) and a synthetic retinoid, Ro 13-6298, suppress TG activity by 85-90% with half-maximal inhibition at 0.1 nM Ro 13-6298 or 1 nM RA. In contrast, the predominant circulating retinoid, retinol, does not inhibit TG activity. The level of type I transglutaminase protein, measured using a type I TG-specific antibody, decreases in parallel with the decrease in activity as does the level of the TG RNA transcript. Cytokeratin K16 decreases more than 20-fold while the level of K7, K8 and K19 increase 5 to 10-fold in the presence of 10 nM RA. Studies using cDNAs encoding K5, K13, K16 and K19 indicate that the RNA transcript levels change in parallel with the change in keratin protein production. Thus, all-trans-retinoic acid suppresses ectocervical epithelial cell differentiation in vitro, a result that suggests an in vivo role for retinoids in regulating cervical cell differentiation.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009353684

Download citation: RISBibTeXText

PMID: 7505756

DOI: 10.1111/j.1432-0436.1993.tb01603.x


Related references

Immortalization by human papillomavirus type 16 alters retinoid regulation of human ectocervical epithelial cell differentiation. Cancer Research 51(15): 3982-3989, 1991

Differential regulation of human ectocervical epithelial cell line proliferation and differentiation by retinoid X receptor- and retinoic acid receptor-specific retinoids. Cell Growth and Differentiation 7(4): 521-530, 1996

Maintenance of in vivo-like keratin expression, sex steroid responsiveness, and estrogen receptor expression in cultured human ectocervical epithelial cells. Endocrinology 126(1): 399-406, 1990

Calcium modulates cornified envelope formation, involucrin content, and transglutaminase activity in cultured human ectocervical epithelial cells. Experimental Cell Research 205(1): 84-90, 1993

Human papillomavirus 16 immortalization of normal human ectocervical epithelial cells alters retinoic acid regulation of cell growth and epidermal growth factor receptor expression. Cancer Research 53(19): 4511-4517, 1993

Retinoid regulation of transglutaminase gene expression in mouse peritoneal macrophages. Journal of Cellular Biochemistry Suppl. (11 Part A): 126, 1987

Down-regulation of keratin 14 gene expression after v-Ha-ras transfection of human papillomavirus-immortalized human cervical epithelial cells. Cancer Research 52(21): 5865-5871, 1992

Novel regulation of keratin gene expression by thyroid hormone and retinoid receptors. Journal of Biological Chemistry 271(3): 1416-1423, 1996

Down-regulation of keratin 4 and keratin 13 expression in oral squamous cell carcinoma and epithelial dysplasia: a clue for histopathogenesis. Histopathology 58(4): 531-542, 2011

AGN193109 is a highly effective antagonist of retinoid action in human ectocervical epithelial cells. Journal of Biological Chemistry 271(21): 12209-12212, 1996

Regulation of tissue transglutaminase gene expression as a molecular model for retinoid effects on proliferation and differentiation. Journal of Cellular Biochemistry 39(3): 293-304, 1989

Role of retinoid receptors in the regulation of mucin gene expression by retinoic acid in human tracheobronchial epithelial cells. Biochemical Journal 338: 351-357, 1999

Gene expression of telomerase related proteins in human normal oral and ectocervical epithelial cells. Oral Oncology 39(5): 445-452, 2003

Retinoid-mediated transcriptional regulation of keratin genes in human epidermal and squamous cell carcinoma cells. Proceedings of the National Academy of Sciences of the United States of America 88(11): 4582-4586, 1991

Regulation of keratin gene expression during differentiation of epidermal and vaginal epithelial cells. Current Topics in Developmental Biology 22: 195-207, 1987