+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Role of chromatin and Xenopus laevis heat shock transcription factor in regulation of transcription from the X. laevis hsp70 promoter in vivo

Role of chromatin and Xenopus laevis heat shock transcription factor in regulation of transcription from the X. laevis hsp70 promoter in vivo

Molecular and Cellular Biology 15(11): 6013-6024

Xenopus laevis oocytes activate transcription from the Xenopus hsp70 promoter within a chromatin template in response to heat shock. Expression of exogenous Xenopus heat shock transcription factor 1 (XHSF1) causes the activation of the wild-type hsp70 promoter within chromatin. XHSF1 activates transcription at normal growth temperatures (18 degrees C), but heat shock (34 degrees C) facilitates transcriptional activation. Titration of chromatin in vivo leads to constitutive transcription from the wild-type hsp70 promoter. The Y box elements within the hsp70 promoter facilitate transcription in the presence or absence of chromatin. The presence of the Y box elements prevents the assembly of canonical nucleosomal arrays over the promoter and facilitates transcription. In a mutant hsp70 promoter lacking Y boxes, exogenous XHSF1 activates transcription from a chromatin template much more efficiently under heat shock conditions. Activation of transcription from the mutant promoter by exogenous XHSF1 correlates with the disappearance of a canonical nucleosomal array over the promoter. Chromatin structure on a mutant hsp70 promoter lacking Y boxes can restrict XHSF1 access; however, on both mutant and wild-type promoters, chromatin assembly can also restrict the function of the basal transcriptional machinery. We suggest that chromatin assembly has a physiological role in establishing a transcriptionally repressed state on the Xenopus hsp70 promoter in vivo.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 009366047

Download citation: RISBibTeXText

PMID: 7565754

DOI: 10.1128/mcb.15.11.6013

Related references

Changes in heat shock protein synthesis and hsp70 gene transcription during erythropoiesis of Xenopus laevis. Developmental Biology 128(1): 111-120, 1988

Examination of the DNA sequence-specific binding properties of heat shock transcription factor in Xenopus laevis embryos. Biochemistry and Cell Biology 70(10-11): 1006-1013, 1992

Heat shock-induced interactions of heat shock transcription factor and the human hsp70 promoter examined by in vivo footprinting. Molecular and Cellular Biology 11(1): 586-592, 1991

Cooperative binding of heat shock transcription factor to the Hsp70 promoter in vivo and in vitro. Journal of Biological Chemistry 269(7): 4804-4811, 1994

In vitro and in vivo analysis of the regulation of a transcription factor gene by thyroid hormone during Xenopus laevis metamorphosis. Molecular Endocrinology 13(12): 2076-2089, 1999

Xenopus NF-Y pre-sets chromatin to potentiate p300 and acetylation-responsive transcription from the Xenopus hsp70 promoter in vivo. Embo Journal 17(21): 6300-6315, 1998

Two transcriptional activators, CCAAT-box-binding transcription factor and heat shock transcription factor, interact with a human hsp70 gene promoter. Molecular and Cellular Biology 7(3): 1129-1138, 1987

In vivo analysis of the Nocturnin promoter using transcription embryonic Xenopus laevis. IOVS 39(4): S236, March 15, 1998

Identification of Xenopus heat shock transcription factor-2: conserved role of sumoylation in regulating deoxyribonucleic acid-binding activity of heat shock transcription factor-2 proteins. Cell Stress & Chaperones 9(2): 214-220, 2004

Regulation of the Xenopus laevis transcription factor IIIA gene during oogenesis and early embryogenesis: negative elements repress the O-TFIIIA promoter in embryonic cells. Developmental Biology 145(2): 241-254, 1991

The transcriptional regulation of xenopus laevis 5s rna genes in chromatin the roles of active stable transcription complexes and histone h 1. Cell 37(3): 903-914, 1984

Developmental regulation of transcription factor AP-2 during Xenopus laevis embryogenesis. Nucleic Acids Research 19(13): 3709-3714, 1991

KRIBB11 inhibits HSP70 synthesis through inhibition of heat shock factor 1 function by impairing the recruitment of positive transcription elongation factor b to the hsp70 promoter. Journal of Biological Chemistry 286(3): 1737-1747, 2011

Preferential transcription of Xenopus laevis ribosomal RNA in interspecies hybrids between Xenopus laevis and Xenopus mulleri. Journal of Molecular Biology 80(2): 217-228, 1973

Fidelity of transcription of Xenopus laevis globin genes injected into Xenopus laevis oocytes and unfertilized eggs. Molecular and Cellular Biology 4(10): 2109-2119, 1984