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Secretory phospholipase A-2 activity is elevated bronchoalveolar lavage fluid after ovalbumin sensitization of guinea pigs



Secretory phospholipase A-2 activity is elevated bronchoalveolar lavage fluid after ovalbumin sensitization of guinea pigs



Journal of Leukocyte Biology 60(6): 704-709



Arachidonic acid (AA), the precursor of eicosanoids, is released from the sn-2 position of phospholipids by both secretory (sPLA-2) and cytosolic in the bronchoalveolar lavage fluid and cells, respectively, in male Hartley guinea pigs sensitized with ovalbumin. sPLA-2 activity was also measured from alveolar macrophages (AM) in culture from sensitized and sensitized animals. There was an increase in sPLA-2 activity and AA content in the lavage fluid following sensitization (18.73 +- 1.33 to 25.74 +- 3.22% hydrolysis and 17.97 +- 12.39 to 44.76 +- 13.37 pmol AA/mL BAL, mean +- SD), which remained elevated but without further increase 4 or 24 h after antigen challenge. AM from unsensitized and sensitizedunchallenged animals did not secrete sPLA-2 activity in culture for 3 h and therefore do not appear to be the cell source of the sPLA-2 activity present in the alveolar lavage fluid following OA sensitization. In contrast to the increase in sPLA-2 in lung lavage fluid, Western blotting for cPLA-2 from lung lavage cells showed no increase 4 or 24 h after antigen challenge compared with sensitization alone. cPLA-2 enzymatic activity of the cytosol fraction of lung lavage cells showed no changes with antigen sensitization or challenge. In summary, intraperitoneal sensitization with ovalbumin in male Hartley guinea pigs caused an increase in both sPLA-2 and AA in bronchoalveolar lavage fluid without a need for antigen challenge. The increased sPLA-2 enzymatic activity following sensitization may be responsible for the elevation of AA in the bronchoalveolar lavage fluid observed after antigen sensitization.

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Accession: 009387956

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PMID: 8975871


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