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Selection and regeneration of hydroxyproline-resistant cell lines of winter barley (Hordeum vulgare L.) for an increased frost tolerance



Selection and regeneration of hydroxyproline-resistant cell lines of winter barley (Hordeum vulgare L.) for an increased frost tolerance



Mitteilungen aus dem Institut fuer Allgemeine Botanik Hamburg 26(0): 163-190



In the present study an in vitro-selection procedure for improvement of frost tolerance in winter barley is described. Embryogenic calli derived from anther cultures of the winter barley variety Igri were plated on solid L3 medium containing the proline analogue hydroxyproline (10-20 mM). After three months of subculture the hydroxyproline-resistant calli as well as control calli were transferred to regeneration medium and plants were regenerated.

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Accession: 009390141

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