+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Selective planting of cationized, haptenized ovalbumin on the rat tubular basement membrane

Selective planting of cationized, haptenized ovalbumin on the rat tubular basement membrane

Virchows Archiv 424(6): 587-591

We developed an experimental protocol for planting exogenous antigens with different molecular weights and charges on the constituents of the renal tubulointerstitium. The cationized antigens were injected selectively into the left renal arteries of Wistar rats. Antigen localization was documented by immunochemistry on frozen sections. Cationized bovine serum albumin (BSA; 68 kDa, isoelectric point = 9.5) localized almost exclusively along the glomerular capillary wall. After application of highly cationic plyethyleneimine, cationized BSA given subsequently was found in a linear distribution along the glomerular capillary wall and along the peritubular capillaries. The fate of highly cationized ovalbumin conjugated with trinitrophenol (TNPOA), subjected to gel filtration to obtain monomers (42 kDa, isoelectric point gt 10) differed; it was deposited in a linear pattern on the tubular basement membrane (TBM) and Bowman's capsule, and remained up to 36 h after injection. Noncationized, monomeric TNP-OA (42 kDa, isoelectric point = 4.6) showed fine granular deposition in the tubular epithelium exclusively. These findings indicate that the barrier of the glomerular BM acts selectively on antigens with different molecular weights. They either settle on the peritubular capillaries, after passing the glomerular, or reach the urinary space, after which they are reabsorbed by the tubular epithelial cells to reach the TBM.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 009392934

Download citation: RISBibTeXText

PMID: 7519953

DOI: 10.1007/bf01069737

Related references

An experimental rat control program Maxwell Street area, Chicago, Illinois, May-October, 1943. Pests 12(1): 8-10, 1944

The antigen of hepatocyte plasma membrane ultrastructural localization in the liver small intestine and primary murine hepatocyte culture detected with monoclonal antibodies. Biologicheskie Membrany (Moscow) 4(2): 208-220, 1987

Induction of immunotolerance to hepatitis B virus antigens during multidrug cancer chemotherapy. European Journal Of Pediatrics. 152(3): 280, 1993

The relationship between tuberous sclerosis cardiac rhabdomyoma and ventricular preexcitation syndromes. Clinical and Investigative Medicine 13(5 Suppl.): C51, 1990

Heparan sulfate proteoglycan from human and equine glomeruli and tubules. International Journal of Biochemistry 20(12): 1391-1400, 1988

The mactar patient preference disability questionnaire an individualized functional priority approach for assessing improvement in physical disability in clinical trials in rheumatoid arthritis. Journal of Rheumatology 14(3): 446-451, 1987

Scanning electron microscopic studies on postcapillary venules and lymphocyte migration into endothelium in rat mesenteric lymph nodes. Acta Medica Universitatis Kagoshimaensis 20(2): 77-92, 1978

Membranous glomerulonephropathy and nephrotic syndrome associated with iatrogenic metallic mercury poisoning in a cat. Veterinary and Human Toxicology 23(1): 1-5, 1981

Renal tubular degeneration after administration of ticrynafen. Archives of Pathology and Laboratory Medicine 104(12): 656, 1980

Tubular lesions in streptozotocin-diabetic rats. Diabetologia 27(1): 32-37, 1984

Guinea pigs immunized with xenogeneic cells skin test in vitro stimulation cyto toxicity of lymph node cells. Cellular Immunology 9(1): 157-162, 1973

Isolation and characterization of the natural gene for human serum albumin. Journal of Cell Biology 87(2 Part 2): 111A, 1980

Renal receptors for mercurial diuretics part 2. Pharmacologist 13(2): 226, 1971

Membranous glomerulo nephritis with extrarenal disorders in children. Medicine (baltimore) 58(3): 219-228, 1979

Experimental anterior uveitis after sub cutaneous injection of feline leukemia sarcoma virus. Investigative Ophthalmology and Visual Science 22(3 Suppl.): 263, 1982