+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Separation and quantitation of stevioside and rebaudioside A in plant extracts by normal-phase high performance liquid chromatography and thin-layer chromatography: A comparison



Separation and quantitation of stevioside and rebaudioside A in plant extracts by normal-phase high performance liquid chromatography and thin-layer chromatography: A comparison



Phytochemical Analysis 5(2): 81-85



Two similar methods, normal phase high performance liquid chromatography and silica gel thin-layer chromatography with densitometry, were applied for the separation and quantitative determination of the diterpene glycosides stevioside and rebaudioside A in the leaves of the plant Stevia rebaudiana Bertoni. Results were compared and found to be very close in all basic features: resolution, accuracy and reproducibility. Thin-layer chromatography was found to be more suitable in case of phytochemical screening of large number of samples.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009397639

Download citation: RISBibTeXText

DOI: 10.1002/pca.2800050207


Related references

Stevia rebaudiana. II. High-performance liquid chromatographic separation and quantitation of stevioside, rebaudioside A and rebaudioside C. Journal of Chromatography 236(2): 523-526, 1982

Optimization of separation of selected active ingredients of plant protection products by gas chromatography, high performance liquid chromatography and thin layer chromatography. Progress in Plant Protection 44(2): 1145-1148, 2004

Separation and quantitation of bromperidol in pharmaceutical preparations by high performance liquid chromatography and high performance thin layer chromatography. Journal of Chromatography 193(2): 255-263, 1980

Separation of natural polar substances by reversed phase high performance liquid chromatography centrifugal thin layer chromatography and droplet countercurrent chromatography. Journal of Chromatography 349(1): 69-76, 1985

Separation of cyto kinins from plant extracts by thin layer chromatography and high pressure liquid chromatography using insoluble poly vinyl pyrrolidone. Plant Physiology (Rockville) Suppl.: 70, 1974

Separation of lepidopterous sex pheromones by reversed-phase thin layer chromatography and high performance liquid chromatography. Agricultural and biological chemistry 50(11): 2935-2937, 1986

Thin layer chromatographic detection and liquid chromatographic determination of stevioside and rebaudioside a in beverages and foods following reverse phase column chromatography. Journal of the Association of Official Analytical Chemists 69(5): 799-802, 1986

Separation and identification of some common isomeric plant triterpenoids by thin-layer chromatography and high-performance liquid chromatography. Journal of Chromatography. a 1216(38): 6662-6670, 2009

A preparative high performance liquid chromatography method for separation of lecithin: comparison to thin-layer chromatography. Journal of Lipid Research 28(5): 596-598, 1987

Separation of 7 alpha and 7 beta methoxycarbonylmethylsteroids by preparative high performance liquid chromatography comparison with thin layer chromatography. Journal of Chromatography 355(2): 427-433, 1986

Comparison of the chromatographic properties of sterols select additional steroids and triterpenoids gravity flow column liquid chromatography thin layer chromatography gas liquid chromatography and high performance liquid chromatography. Journal of Chromatography 452: 377-398, 1988

Overpressured layer chromatography in comparison with thin-layer and high-performance liquid chromatography for the determination of coumarins with reference to the composition of the mobile phase. Journal of Chromatography A 670(1-2): 191-198, 1994

A note on the separation of natural mixtures of bacterial ubi quinones using reverse phase partition thin layer chromatography and high performance liquid chromatography. Journal of Applied Bacteriology 51(1): 129-134, 1981

Separation and purification of an endogenous inhibitor of sodium pump from chansu by thin-layer chromatography and reversed-phase high performance liquid chromatography. Se Pu 19(6): 555-557, 2001

Comparison of liquid-liquid extraction-thin layer chromatography with solid-phase extraction-high-performance thin layer chromatography in detection of urinary morphine. Journal of Biomedical Research 25(5): 362-367, 2011