+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Sequence of the cDNA and gene coding for ribosomal protein S1 of Xenopus laevis

Sequence of the cDNA and gene coding for ribosomal protein S1 of Xenopus laevis

Gene 154(2): 145-151

The cloning and complete sequencing of one of the two gene copies coding for ribosomal protein (r-protein) S1 in Xenopus laevis and of the corresponding cDNA are reported. The comparison of the sequence of this cDNA (S1b) with the other (S1a) previously reported, reveals that, while the two DNA sequences have diverged somewhat, the amino-acid sequences are mostly unchanged. The two gene copies are apparently expressed at comparable levels, since the two corresponding mRNAs are similarly represented in oocyte poly(A) RNA. The S1b gene has a total length of about 12,000 nt and is composed of seven exons and six introns. By primer extension, it has been determined that the transcription start point is located in a pyrimidine-rich tract, as observed for all r-protein genes of X. laevis and other vertebrates so far analyzed. A computer analysis of the S1 sequence has shown the presence of a 150-nt sequence repeated in introns 3, 5 and 6, which is homologous to the one reported in the first intron of mammalian r-protein S3 gene. Furthermore, a 130-nt sequence is tandemly repeated 2.5 times at each of the two sites near the beginning and near the end of the first intron.

(PDF emailed within 1 workday: $29.90)

Accession: 009399782

Download citation: RISBibTeXText

PMID: 7890156

Related references

Sequence of the gene coding for ribosomal protein S8 of Xenopus laevis. Gene (Amsterdam) 132(2): 255-260, 1993

Xenopus laevis ribosomal protein S1a cDNA sequence. Nucleic Acids Research 19(8): 1943-1943, 1991

Sequence of a cDNA encoding rat ribosomal protein homologous to Xenopus laevis ribosomal protein S8. Nucleic Acids Research 19(6): 1339-1339, 1991

A complete cDNA sequence for ribosomal protein S6 of Xenopus laevis. Nucleic Acids Research 21(21): 4981-4981, 1993

Nucleotide sequence of a hamster cDNA highly homologous to the Xenopus laevis S19 ribosomal protein. Nucleic Acids Research 18(13): 3997-3997, 1990

Xenopus laevis ribosomal protein L22: Full-length cDNA sequence and expression analysis. Gene (Amsterdam) 154(2): 199-203, 1995

Sequence and expression analysis of a novel Xenopus laevis cDNA that encodes a protein similar to bacterial and chloroplast ribosomal protein L24. Gene (Amsterdam) 235(1-2): 13-18, July 22, 1999

Sequence of the ribosomal protein complementary dna of drosophila melanogaster homologous to the l1 ribosomal protein gene of xenopus laevis. Nucleic Acids Research 17(1): 456, 1989

Molecular cloning and sequence determination of a cDNA coding for the alpha-subunit of a Go-type protein of Xenopus laevis oocytes. Febs Letters 244(1): 188-192, 1989

Sequence of the ribosomal protein cDNA of D. melanogaster homologous to the L1 ribosomal protein gene of X. laevis. Nucleic Acids Research 17(1): 456-456, 1989

Nucleotide sequence of the L1 ribosomal protein gene of Xenopus laevis: remarkable sequence homology among introns. Embo Journal 4(13a): 3483-3488, 1985

The nucleotide sequence of the ribosomal protein L14 gene of Xenopus laevis. Nucleic Acids Research 15(4): 1870-1872, 1987