EurekaMag
+ Translate
+ Most Popular
Spermatogenic and steroidogenic impairment after chromium treatment in rats
Prediction of enthalpy of formation based on refined crystal structures of multisite compounds; Part 1, Theories and examples
Intrastromal photorefractive keratectomy for myopia by Nd:YLF picosecond laser
Teachers' beliefs about the role of prior language knowledge in learning and how these influence teaching practices
Classification and genesis of submarine iron-manganese deposits
Insecta a Cl. G. N. Potanin in China et in Mongolia novissime lecta. XVII. Hemiptera-Heteroptera
On the occurrence of the splendid genus Machaerothrix Haupt in Tsushima, with some remarks on the genus (Hymenoptera, Pompilidae)
Enamel craze lines
Gynecologic examination of the child and the adolescent
Periosteal osteoclasts, skeletal development and ageing
Fibrodysplasia ossificans progressiva in a Nigerian: a case report
The chronically ill patient with anorexia nervosa development, phenomenology, and therapeutic considerations
Kummelonautilus taiwanum sp. nov. (Nautilida) from the early Miocene Shihmen Formation, Chungliao, Nantou, west-central Taiwan
Efficacy of Droncit against Dipylidium caninum in dogs
Pierids of the genus Euchloe Klots (Lepidoptera, Pieridae) from Siberia and the Far East.
C5A anaphylatoxin and its seven transmembrane-segment receptor
Failure to practice evidence-based medicine: why do physicians not treat patients with heart failure with angiotensin-converting enzyme inhibitors?
Empirical comparisons of proportional hazards regression, Poisson regression, and logistic regression in the analysis of occupational cohort data
Pollination of flowers of sweet and sour cherries
Isopodi terrestri raccolti nell' Estremo Oriente
Morphometric observations on Dipylidium caninum from dogs and cats in Austria
Chelation effectiveness of zinc proteinates demonstrated
Alopecia--a psychosomatic disease picture? I. Review of the literature
Monitoring equity in health and healthcare: a conceptual framework
Hemodynamic evaluation of the Biocor heterologous prosthesis

Sequence, structure and evolution of the ecdysone-inducible Lsp-2 gene of Drosophila melanogaster


Sequence, structure and evolution of the ecdysone-inducible Lsp-2 gene of Drosophila melanogaster



European Journal of Biochemistry 245(1): 191-198



ISSN/ISBN: 0014-2956

PMID: 9128741

DOI: 10.1111/j.1432-1033.1997.00191.x

The Lsp-2 gene encodes a major larval serum protein (hexamerin) of Drosophila melanogaster. Transcription of Lsp-2 is controlled by 20-hydroxyecdysone. Here we report the analysis of the structure of the Lsp-2 gene including the adjacent 5' and 3' sequences. In contrast to all other known hexamerin genes, Lsp-2 does not contain an intron. The Lsp-2 mRNA measures 2312 bases, as deduced from experimental determination of the transcription-start and stop sites and conceptual translation results in a 718 amino acid hexamerin subunit, including a 21-amino-acid signal peptide. While the calculated molecular mass of the native 697-amino-acid subunit is 83.5 kDa, mass spectrometry gave a value of 74.5 kDa. We detected in the Lsp-2 gene a 2052-bp antisense ORF that probably does not code for any protein. An unusual accumulation of rarely used codon triplets was found at the 5' and 3' ends of the Lsp-2 ORF. The calculated secondary structure matches well with that of arthropod hemocyanins. Electron micrographs show for LSP-2 hexamers a cubic shape, which can not be easily reconciled with its hexameric structure. Phylogenetic analysis revealed that LSP-2 diverged from the LSP-1-like hexamerins after separation of the Diptera from other insect orders.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009400200

Download citation: RISBibTeXText

Related references

Structure of the ecdysone-inducible P1 gene of Drosophila melanogaster. Journal of Molecular Biology 214(2): 359-372, 1990

Genetic and molecular analysis of an ecdysone inducible gene from drosophila melanogaster. Genetics Part 2: S15-16, 1984

Transformation mapping of the regulatory elements of the ecdysone-inducible P1 gene of Drosophila melanogaster. Molecular and Cellular Biology 11(5): 2913-2917, 1991

Hormonal and developmental specificities of transcription lie within a 1.5 kilobase region 5' to the ecdysone inducible drosophila melanogaster p 1 gene. EMBO (European Molecular Biology Organization) Journal 5(3): 583-588, 1986

A member of the steroid hormone receptor gene family is expressed in the 20-OH-ecdysone inducible puff 75B in Drosophila melanogaster. Nucleic Acids Research 17(18): 7167-7178, 1989

Characterization of a putative transcription factor gene expressed in the 20-OH-ecdysone inducible puff 74EF in Drosophila melanogaster. Nucleic Acids Research 17(12): 4455-4464, 1989

The promoter of DNA puff gene II/9-1 of Sciara coprophila is inducible by ecdysone in late prepupal salivary glands of Drosophila melanogaster. Cell Regulation 2(11): 875-888, 1991

Sequence, structure and evolution of the gene coding for sn-glycerol-3-phosphate dehydrogenase in Drosophila melanogaster. Nucleic Acids Research 17(21): 8553-8567, 1989

Sequence and expression of IMP-L1, an ecdysone-inducible gene expressed during Drosophila imaginal disc morphogenesis. Developmental Genetics 13(5): 331-344, 1992

Structure of the Eip28/29 gene, an ecdysone-inducible gene from Drosophila. Journal of Molecular Biology 189(4): 617-631, 1986

The E23 early gene of Drosophila encodes an ecdysone-inducible ATP-binding cassette transporter capable of repressing ecdysone-mediated gene activation. Proceedings of the National Academy of Sciences of the United States of America 97(17): 9519-9524, 2000

Interspecific comparisons of the structure and regulation of the Drosophila ecdysone-inducible gene E74. Genetics 127(3): 535-543, 1991

Sequential gene activation by ecdysone in polytene chromosomes of Drosophila melanogaster. I. Dependence upon ecdysone concentration. Developmental Biology 35(1): 47-61, 1973

Sequential gene activation by ecdysone in polytene chromosomes of Drosophila melanogaster. III. Consequences of ecdysone withdrawal. Developmental Biology 54(2): 241-255, 1976

Control elements of ecdysone inducible genes of drosophila melanogaster. Journal of Cellular Biochemistry Suppl. (10 Part C): 63, 1986