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Sequential effects of an oral enzyme combination with rutosid in different in vitro and in vivo models of inflammation



Sequential effects of an oral enzyme combination with rutosid in different in vitro and in vivo models of inflammation



International Journal of Immunotherapy 13(3-4): 139-145



The antiedematous and antiinflammatory potential of the proteolytic enzymes trypsin, chymotrypsin, bromelin and papain, and the flavonoid rutosid were investigated in the following models: a) in vivo carrageenin-induced rat paw edema; protein-induced paw edema; arachidonic acid-induced ear edema; passive cutaneous anaphylaxis; histamine-induced skin reaction; and, b) in vitro arachidonic acid-induced platelet aggregation; protamine-induced degranulation; and, PAF-induced platelet aggregation, Bromelin, chymotrypsin and papain demonstrated a strong antiinflammatory potential and were highly effective in the carrageenin-induced paw edema model. The effects were strongly correlated to the applied dose. Only papain demonstrated an antiexsudative effect (protein-induced ear edema). Bromelin showed a modulatory effect with regard to the arachidonic acid metabolism. Both bromelin and papain demonstrated a strong antihistaminic effect, which may be due to an inhibition of degranulation (antianaphylactic potency). An inhibitory potency could be observed for rutosid and trypsin with regard to the degranulation of mast cells. No effects of the tested compounds were observed in the models of arachidonic acid-induced ear edema and PAF-induced platelet aggregation. A combination of these enzymes is at an advantage to show sequential synergy in inflammatory processes.

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Accession: 009401064

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