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Serum protein determination by high-performance gel-permeation chromatography


Serum protein determination by high-performance gel-permeation chromatography



Journal of Chromatography. B Biomedical Sciences and Applications 696(1): 19-23



ISSN/ISBN: 1387-2273

PMID: 9300904

A general high-performance gel-permeation chromatography (HPGPC) method was developed to determine protein in human serum with improved sensitivity and speed. The optimum UV wavelength for protein detection was found to be 210 nm, by comparing the protein values obtained by varying the UV wavelength of the HPLC detection system with the protein values obtained from spectrophotometric protein assays, i.e., the bicinchoninic acid (BCA) method and the biuret method. The analysis time was less than 1 min. Since this HPGPC serum protein assay method is simple and rapid, it is expected to be particularly well adapted for use in clinical laboratories.

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Accession: 009406383

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