+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity



Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity



Biochemical Journal 293: 835-842



Stimulation of the human T-cell line, Jurkat, by a monoclonal antibody (mAb) directed against the CD28 molecule leads to sustained increases in intracellular levels of Ca-2+ ((Ca-2+)-i); the initial rise in Ca-2+ comes from internal stores, followed by Ca-2+ entry into the cells. The CD28 molecule also appears to activate polyphosphoinositide (InsPL)-specific phospholipase C (PLC) activity in Jurkat cells, as demonstrated by PtdInsP-2 breakdown, InsP-3 and 1,2-diacylglycerol generation and PtdIns resynthesis. We also observed that interleukin-2 (IL2) production induced via CD28 triggering was sensitive to a selective protein kinase C inhibitor. Of the four other anti-CD28 mAbs (CD28.2, CD28.4, CD28.5, CD28.6) tested, only one (CD28.5) was unable to generate any InsPL-specific PLC or IL2 secretion. However, the cross-linking of cell-bound CD28.5 with anti-mouse Ig antibodies led to an increase in (Ca-2+)-i. CD28-molecule clustering in itself appears to be a sufficient signal for induction of PLC activity.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009415196

Download citation: RISBibTeXText

PMID: 8394695

DOI: 10.1042/bj2930835


Related references

Inositol Phospholipid Metabolism in Arabidopsis. Characterized and Putative Isoforms of Inositol Phospholipid Kinase and Phosphoinositide-Specific Phospholipase C. Plant Physiology 130(1): 22-46, 2002

Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C. Plant Physiology 130(1): 22-46, 2002

Monitoring Inositol-Specific Phospholipase C Activity Using a Phospholipid FlashPlate(R). Journal of Biomolecular Screening 4(3): 151-155, 1999

Monitoring Inositol-Specific Phospholipase C Activity Using a Phospholipid FlashPlate(R). Journal of Biomolecular Screening 4(3): 151-155, 1999

Histamine-stimulated phospholipase C signalling in the adrenal chromaffin cell: effects on inositol phospholipid metabolism and tyrosine hydroxylase phosphorylation. Clinical and Experimental Pharmacology & Physiology 24(8): 624-631, 1997

Mitogenic stimulation of human B lymphocytes via the inositol phospholipid 'dual pathway' of signalling requires persistent activation of both second messenger arms. Immunology 65(3): 493-494, 1988

Tissue and cell type specific expression of messenger rna for four types of inositol phospholipid specific phospholipase c. Biochemical & Biophysical Research Communications 164(1): 406-412, 1989

Tissue- and cell type-specific expression of mRNAs for four types of inositol phospholipid-specific phospholipase C. Biochemical and Biophysical Research Communications 164(1): 406-412, 1989

Signalling via CD28 involves association with and activation of phosphoinositide 3-kinase. Journal of Cellular Biochemistry Suppl. 0(18D): 433, 1994

Activation of inositol phospholipid-specific phospholipase C by P2-purinergic receptors in human phagocytic leukocytes. Role of pertussis toxin-sensitive G proteins. Annals of the new York Academy of Sciences 603: 227-44; Discussion 244-5, 1990

Collapsin signalling involves phospholipase A-2 activation. Society for Neuroscience Abstracts 23(1-2): 600, 1997

T lymphocyte activation via the CD28 pathway involves the activation of protein kinase C and provides essential costimulation for superantigen induced mitogenesis. Blood 82(10 Suppl. 1): 245A, 1993

Costimulation of T cell receptor/CD3-mediated activation of resting human CD4+ T cells by leukocyte function-associated antigen-1 ligand intercellular cell adhesion molecule-1 involves prolonged inositol phospholipid hydrolysis and sustained increase of intracellular Ca2+ levels. Journal of Immunology 149(12): 3872-3880, 1992

A transfected human muscarinic receptor that regulates the inositol phospholipid second messenger pathway can induce t cell activation. Clinical Research 38(2): 290A, 1990

Transmembrane signalling via the T11-dependent pathway of human T cell activation. Evidence for the involvement of 1,2-diacylglycerol and inositol phosphates. European Journal of Immunology 17(1): 55-60, 1987