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Simple high-performance liquid chromatographic method for the quantitation of 5-fluorouracil in human plasma



Simple high-performance liquid chromatographic method for the quantitation of 5-fluorouracil in human plasma



Journal of Chromatography B Biomedical Applications 677(2): 380-383



A simple, rapid, specific and sensitive high-performance liquid chromatography method has been developed for quantitation of 5-fluorouracil (5-FU) in human plasma. The method involves deproteinization of a small sample volume of plasma (150 Al) followed by HPLC on a cation-exchange resin column, Aminex HPX-87H (300 times 7.8 mm I.D.), preceded by a similar guard cartridge with UV detection at 265 nm. This method allows a good separation of 5-FU with a retention time of 24 min and a detection limit at 25 ng/ml. The calibration curve was linear from 25 to 2000 ng/ml. The coefficient of variation was ltoreq 4.4% for within-day reproducibility and ltoreq ltoreq 9.5% for day-to-day reproducibility.

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Accession: 009417582

Download citation: RISBibTeXText

PMID: 8704945

DOI: 10.1016/0378-4347(95)00352-5



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