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Site-specific spontaneous deletions in three genome regions of a temperate Streptococcus thermophilus phage

Site-specific spontaneous deletions in three genome regions of a temperate Streptococcus thermophilus phage

Virology 219(1): 96-104

ISSN/ISBN: 0042-6822

PMID: 8623559

DOI: 10.1006/viro.1996.0226

Site-specific spontaneous deletions were observed with high frequency in three regions of the genome of the temperate Streptococcus thermophilus phage vphi-SFi21. Deletion sizes were 750 bp (type 1), 2.7 kb (type 2), and 1 kb (type 3). Combinations of types 1 and 3 and 2 and 3 were observed. The mutants grew lytically although with reduced burst sizes. Type 2 mutants lost the capacity to lysogenize host cells. Upon serial passage, the deletion mutants overgrew the wild-type phage. No direct or inverted DNA repeats were associated with type 1 or 2 deletion sites. Several independent phage isolates showed deletions at identical nucleotide positions, suggesting a site-specific recombination system. Sequencing of an XbaI restriction fragment covering the type 1 deletion predicted a single long open reading frame (ORF) showing a high degree of amino acid similarity with two proteins from bacteriophage P1 implicated in its immunity control (KilA, Ant). Type 1 deletion leads to a loss of the conserved C-terminal part of this ORF.

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Accession: 009423099

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