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Size exclusion HPLC for determining the purity and molecular homogeneity of recombinant Hepatitis B virus surface antigen (AgsHBr)

Size exclusion HPLC for determining the purity and molecular homogeneity of recombinant Hepatitis B virus surface antigen (AgsHBr)

Biotecnologia Aplicada 11(1): 86-90

A precise and rugged high performance size exclusion chromatography method for the control of purity and molecular homogeneity of recombinant Hepatitis B surface antigen was developed and validated on TSK G5000PW (7.5 times 600 mm). The chromatographic method showed a good resolution for the separation of the antigen particle (22-27 nm) from antigen particles with smaller size. The precision between runs and interlaboratories was under 1%.

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