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Size-exclusion chromatography of cellulose and chitin using lithium chloride-N,N-dimethylacetamide as a mobile phase



Size-exclusion chromatography of cellulose and chitin using lithium chloride-N,N-dimethylacetamide as a mobile phase



Journal of Chromatography 635(2): 334-337



Distributions of molecular mass of cellulose and chitin were determined by size-exclusion chromatography (SEC) using 5% (w/w) lithium chloride-N,N-dimethylacetamide as an eluent. The peak positions in SEC patterns of the cellulose samples used corresponded well to their molecular masses, which were measured by a viscometric method using cupriethylenediamine. Thus, the packed gel consisting of styrene-divinylbenzene copolymer was found to be applicable to the SCE analysis of cellulose and chitin, when 5% (w/w) lithium chloride-N,N-dimethylacetamide was used as the solvent for polysaccharides and the eluent. The elution patterns obtained in this SEC system indicated that the molecular mass of commercial chitins prepared from crab shell is much higher than that of cotton, and that the chitins have two peaks of distribution of molecular mass.

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Accession: 009423900

Download citation: RISBibTeXText

DOI: 10.1016/0021-9673(93)80377-k


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