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Solid-state spin trapping of the hydroxyl radical formed by gamma-irradiation in ice and the scavenging effect of sodium L-ascorbate


Solid-state spin trapping of the hydroxyl radical formed by gamma-irradiation in ice and the scavenging effect of sodium L-ascorbate



Bioscience Biotechnology and Biochemistry 60(12): 1971-1975



ISSN/ISBN: 0916-8451

DOI: 10.1271/bbb.60.1971

Spin trapping of the hydroxyl (OH) radical formed by gamma-irradiation in ice was performed by using 5,5-dimethyl pyrroline N-oxide (DMPO) as a trapping agent. A frozen aqueous solution of DMPO was gamma-irradiated at -70 degree C for three days, and ESR spectra were then measured at room temperature as a function of time after melting. A strong four-line absorption characteristic of the spin adduct (DMPO-OH) was observed and the intensity decreased with time, while no signal was observed when gamma-irradiation was carried out at room temperature. The intensity of DMPO-OH increased linearly with radiation dose, but it was not changed by the standing time at -70 degree C after gamma-irradiation, suggesting that the OH radical formed by gamma-irradiation in ice was trapped by DMPO in the frozen state, DMPO-OH being stable for more than three days in contrast to the case at room temperature. The quantity of DMPO-OH was decreased in the presence of sodium L-ascorbate. The analysis of the intensity change shows that this solid-state spin trapping technique is useful to measure separately the OH radical scavenging and DMPO-OH scavenging abilities of antioxidants.

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Accession: 009431149

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