+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Some aspects of melanin formation of melanocytes cultured on collagen-coated microcarrier beads

Some aspects of melanin formation of melanocytes cultured on collagen-coated microcarrier beads

Pigment Cell Research 8(2): 89-96

Melanocytes cultured on collagen-coated Cytodex 3 microcarrier Sephadex beads caused remarkable pigmentation of the beads during the period of culture when optimal density was reached. Electron microscopy of melanocytes on the microcarriers revealed that the cells and their dendrites invaginate into the microcarrier surface layer. Removal of the cells by trypsinization showed that some pigment granules were left on the carrier surface and within the cavities present on the microcarrier surface. In order to investigate whether the pigmentation of the microcarriers could be a result of indole intermediates of melanogenesis present in the culture medium, extracts were studied by gas chromatography/mass spectrometry for the presence of these compounds. Two compounds (5,6-dihydroxyindole-2-carboxylic acid and 6-hydroxy-5-methoxyindole-2-carboxylic acid) so far have been identified in the medium extracts. Results indicate that microcarrier culture of melanocytes can serve as an interesting model for electron microscopy studies of melanocytes with regard to pigmentation and cell attachment.

(PDF emailed within 0-6 h: $19.90)

Accession: 009434333

Download citation: RISBibTeXText

PMID: 7659682

DOI: 10.1111/j.1600-0749.1995.tb00647.x

Related references

Large-scale cultivation of human melanocytes using collagen-coated Sephadex beads (cytodex 3). Journal of Investigative Dermatology 92(1): 18-21, 1989

Variations in melanin formation by cultured melanocytes from different skin types. Archives of Dermatological Research 290(6): 342-349, 1998

Use of microcarrier beads to facilitate epithelial outgrowth inside collagen gel. Methods in Cell Science 7(2): 73-75, 1982

Does rapamycin induce melanin formation? An in vitro study assessing the effect of rapamycin on normal cultured melanocytes. Indian Journal of Dermatology, Venereology and Leprology 2019, 2019

Response of intraventricular macrophages to crotoxin-coated microcarrier beads injected into the lateral ventricle of postnatal rats. Journal of Anatomy 168: 63-72, 1990

Studies on anf processing and release from adult rat cardiocytes cultured on microcarrier beads. FASEB Journal 5(5): A1021, 1991

Flow-induced modulation of the permeability of endothelial cells cultured on microcarrier beads. Journal of Cellular Physiology 168(2): 403-411, 1996

The use of cytodex microcarrier beads in patch-clamp studies on cultured epithelial cells. Pflugers Archiv 413(1): 90-92, 1988

Release of catecholamines from superfused bovine adrenal chromaffin cells cultured on microcarrier beads. Journal of Neurochemistry 46(5): 1473-1477, 1986

Sustained high-yield production of recombinant proteins in transiently transfected COS-7 cells grown on trimethylamine-coated (hillex) microcarrier beads. Biotechnology Progress 19(1): 9-13, 2003

Phosphorus-31 nuclear magnetic resonance study of the C6 glioma cell line cultured on microcarrier beads. Brain Research 493(1): 175-178, 1989

Reversible beta receptor modulation of the permeability of an endothelial cell layer cultured on microcarrier beads. Federation Proceedings 46(3): 500, 1987

Differentiation of mammalian skeletal muscle cells cultured on microcarrier beads in a rotating cell culture system. Medical & Biological Engineering & Computing 38(5): 583-590, 2000

Expression of VEGF by human keratinocytes cultured on microcarrier beads is modulated by wound fluid and hydrogen peroxide. 2007

Methimazole is an inhibitor of melanin synthesis in cultured B16 melanocytes. Journal of Investigative Dermatology 122(5): 1338-1341, 2004