+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Specific cytogenetic aberrations in two novel human prostatic cell lines immortalized by human papillomavirus type 18 DNA

Specific cytogenetic aberrations in two novel human prostatic cell lines immortalized by human papillomavirus type 18 DNA

Cancer Genetics & Cytogenetics 99(2): 108-115

Using chromosome banding and fluorescence in situ hybridization (FISH) with painting probes, sequential cytogenetic analysis was performed of two novel prostate cell lines, PZ-HPV-7 and CA-HPV-10, established by human papillomavirus (HPV) 18 DNA transformation. PZ-HPV-7 originates from a normal diploid prostate epithelial cell strain. PZ-HPV-7 progressed from an initial diploid to a hypertetraploid chromosome number with a relative gain of chromosomes 5 and 20 (7 to 8 copies each). Structural changes were limited; 3p- (2 copies), 3q- (1 copy), and possibly a der(16p;12q). CA-HPV-10 originates from an epithelial cell strain derived from a high-grade human prostate cancer specimen, which showed several karyotypic abnormalities including an extra Y chromosome and double minutes (dmin). In early passage the karyotype of CA-HPV-10 appeared unstable with a decreasing number of cells exhibiting dmin. In late passage the dmin were replaced by a large homogeneously staining region (hsr) on 9p+ marker. The hsr was shown by FISH to be of chromosome 1 origin. The modal number was mainly hypertriploid (72, range 69 to 75). Loss of Y was remarkable (0 to 1 copy). Consistent markers included two copies each of del(1)(q12q31) and der(9)t(1;9)(?;p22), and one der(11)t(4;11) (?;q21). HPV type 18 genomic integration sites were identified on 1p for PZ-HPV-7 and on the 9p+ marker for CA-HPV-10. In conclusion, both PZ-HPV-7 and CA-HPV-10 showed clonal cytogenetic changes. These two cell lines constitute a novel in vitro model to study the mechanisms involved in human prostate carcino-genesis.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 009442160

Download citation: RISBibTeXText

PMID: 9398864

DOI: 10.1016/s0165-4608(97)00207-0

Related references

Cytogenetic analysis of eight human papillomavirus immortalized human keratinocyte cell lines. International Journal of Cancer 44(6): 1124-1131, 1989

Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18. Carcinogenesis 18(6): 1215-1223, 1997

Expression of prostatic factors measured by reverse transcription polymerase chain reaction in human papillomavirus type 18 deoxyribonucleic acid immortalized prostate cell lines. Urology. 51(4): 657-662, Il, 1998

Elevated wild-type p53 protein levels in human epithelial cell lines immortalized by the human papillomavirus type 16 E7 gene. Virology 198(1): 169-174, 1994

Viral transcription in human keratinocyte cell lines immortalized by human papillomavirus type-16. Virology 183(1): 331-342, 1991

Distinct squamous phenotypes of human ectocervical cell lines immortalized by human papillomavirus type 16. Molecular & Cellular Differentiation 2(2): 141-157, 1994

Retinoid regulation of cell differentiation in a series of human papillomavirus type 16-immortalized human cervical epithelial cell lines. Carcinogenesis 16(2): 375-381, 1995

Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part I. Cell markers and immortalized nontumorigenic cell lines. Prostate 29(6): 386-394, 1996

Expression and splicing patterns of human papillomavirus type 16 mrnas in pre cancerous lesions and carcinomas of the cervix in human keratinocytes immortalized by hpv 16 and in cell lines established from cervical cancers. International Journal of Cancer 50(3): 356-364, 1992

Molecular and cytogenetic analysis of immortalized human primary keratinocytes obtained after transfection with human papillomavirus type 16 DNA. Oncogene 1(3): 251-256, 1987

A cell-type-specific transcription enhancer of type 16 human papillomavirus (HPV 16)-P97 promoter is defined with HPV-associated cellular events in human epithelial cell lines. Virology 195(2): 500-510, 1993

The immortalized cell lines induced by human papillomavirus type 16 (HPVS 16) YY1 binding-site mutants have some characteristics of transformed cells. Zhonghua Shi Yan He Lin Chuang Bing du Xue Za Zhi 14(3): 205-208, 2001