+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Specific pattern of unconjugated bilirubin during fasting can identify constitutional hyperbilirubinemia

Specific pattern of unconjugated bilirubin during fasting can identify constitutional hyperbilirubinemia

Italian Journal of Gastroenterology 25(7): 375-379

The pattern of individual bilirubin pigments during a 24 hour caloric restriction (400 Kcal) was investigated in three groups of patients: one group with constitutional hyperbilirubinaemia (n = 29), another with microcytaemia and signs of haemolysis (n = 15) and the third consisting of patients without signs of hepatic disease (n = 11). The different bilirubin fractions (unconjugated, mono- and di-conjugated) were separated as methylated tetrapyrroles by t.l.c. following alkaline methanolysis. In all patients fasting induced an enhancement of the unconjugated bilirubin while the concentration of mono- and di-methyl esters of conjugated bilirubins remained within the normal range. When a pre-fasting concentration of total bilirubin equal to 1.2 mg/dl was used as a discriminating point, two different patterns in the fasting-induced increase in unconjugated bilirubin were identified. An increase of more than 1 mg/dl was found in 30 patients and 28 of these had constitutional hyperbilirubinaemia as diagnosed by exclusion of other disorders. The group with an increase of less than 1 mg/dl was composed of 25 patients, only one of whom had constitutional hyperbilirubinaemia: 14 were affected by microcytaemia and 10 were outpatients without signs of disease. The "fasting-induced increase in unconjugated bilirubin" has a specificity of 78%, a sensitivity of 84% and a positive and negative predictive value of 85 and 76%, respectively, for the diagnosis of constitutional hyperbilirubinaemia.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 009442930

Download citation: RISBibTeXText

PMID: 8280899

Related references

Association of UGT1A1 mutation with fasting hyperbilirubinemia and constitutional unconjugated hyperbilirubinemia. Hepatology 30(4 Part 2): 525A, 1999

Constitutional hyperbilirubinemia with unconjugated bilirubin in the serum and lipochrome-like pigment granules in the liver. Annals of Internal Medicine 56: 308-314, 1962

Constitutional Hyperbilirubinemia with Unconjugated Bilirubin in the Serum and Pigment Deposition in the Liver. Report of a Case. American Journal of Digestive Diseases 9: 160-169, 1964

Intestinal excretion of unconjugated bilirubin in man and rats with inherited unconjugated hyperbilirubinemia. Pediatric Research 42(2): 195-200, 1997

Caffeine clearance in subjects with constitutional unconjugated hyperbilirubinemia. Italian Journal of Gastroenterology 27(3): 129-132, 1995

Elimination of falsely elevated direct bilirubin values due to unconjugated hyperbilirubinemia a total and direct bilirubin assay for the technicon ra 1000. Clinical Chemistry: 1199, 1986

Intestinal excretion of bilirubin in unconjugated hyperbilirubinemia. Journal of Hepatology 16(Suppl. 1): S62, 1992

Non enzymatic alkaline hydrolysis and isomerization of pure bilirubin monoglucuronide to unconjugated bilirubin in model bile systems and the coprecipitation of bilirubin monoglucuronide and unconjugated bilirubin. Hepatology 4(5): 1065, 1984

Two cases of constitutional unconjugated hyperbilirubinemia with marked retention of indocyanine green. Acta Medica Okayama 38(6): 565-567, 1984

A constitutional unconjugated hyperbilirubinemia combined with indocyanine green intolerance: a new functional disorder?. Hepatology 1(4): 319-324, 1981

Heterogeneity of bilirubin in familial unconjugated hyperbilirubinemia, Gilbert's syndrome. Jinrui Idengaku Zasshi. Japanese Journal of Human Genetics 19(3): 203-216, 1974

Effect of yin zhi huang on bilirubin excretion in animals with unconjugated hyperbilirubinemia. Zhong Xi Yi Jie he Za Zhi 9(5): 289-91 262, 1989

Fractional disappearance rates of caffeine and indocyanine green in subjects with constitutional unconjugated hyperbilirubinemia. Journal of Hepatology 11(Suppl. 2): S115, 1990

Common variants in the SLCO1B3 locus are associated with bilirubin levels and unconjugated hyperbilirubinemia. Human Molecular Genetics 18(14): 2711-2718, 2009

Intestinal excretion is a major route of bilirubin disposition in unconjugated hyperbilirubinemia. Hepatology 14(4 Part 2): 230A, 1991