Specificity of PS integrin function during embryogenesis resides in the alpha subunit extracellular domain
Martin-Bermudo, M.D.; Dunin-Borkowski, O.M.; Brown, N.H.
EMBO Journal 16(14): 4184-4193
1997
ISSN/ISBN: 0261-4189
PMID: 9250662
DOI: 10.1093/emboj/16.14.4184
Accession: 009443512
We tested the ability of different integrin alpha subunits to substitute for each other during embryonic development. Two alpha subunits, which form heterodimers with the same beta PS subunit, are expressed in complementary tissues in the Drosophila embryo, with alpha PS1 expressed in the epidermis and endoderm, and alpha PS2 expressed in the mesoderm. As a result the two integrin heterodimers are present on opposite surfaces at sites of interaction between the mesoderm and the other cell layers where they are required for normal development. Using the GAL4 system we are able to rescue fully the embryonic lethality of an alpha PS2 null mutation with a UAS-alpha PS2 transgene, but only partially with a UAS-alpha PS1 gene, due to partial rescue of both muscle and midgut phenotypes. Similarly we are able to rescue the embryonic/first instar larval lethality of an alpha PS1 null mutation gene with UAS-alpha PS1, but only partially with UAS-alpha PS2. Each UAS-alpha gene, when it contains the cytoplasmic domain from the other alpha subunit, maintains an equivalent ability to rescue its own mutation and cannot fully rescue a mutation in the other alpha. We conclude that the two alpha subunits are not equivalent and have distinct functions which reside in the extracellular domains.