Structural and functional analysis of the 5'-flanking region of the human insulin-like growth factor binding protein (IGFBP) -4 gene

Qin, X.; Morales, S.; Lee, K.W.; Boonyaratanakornkit, V.; Baylink, D.J.; Mohan, S.; Strong, D.D.

Biochimica et Biophysica Acta 1350(2): 136-140


ISSN/ISBN: 0006-3002
PMID: 9048882
DOI: 10.1016/s0167-4781(96)00220-5
Accession: 009464171

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More than 3 kb of the human (h)IGFBP-4 gene 5'-flanking region was sequenced and assessed for promoter activity. The hIGFBP-4 promoter resides within a CpG island and demonstrates strong basal activity in human osteoblast-like osteosarcoma and COS-7 monkey kidney cells. Transient transfection of cells with hIGFBP-4 promoter-linked deletion constructs demonstrated that multiple cooperating cis-acting elements within 836 bp of the 5'-flanking region contributed to overall promoter strength.