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Structural determination of the capsular polysaccharide produced by Klebsiella pneumoniae serotype K40. NMR studies of the oligosaccharide obtained upon depolymerisation of the polysaccharide with a bacteriophage-associated endoglycanase

Cescutti, P.; Toffanin, R.; Kvam, B.J.; Paoletti, S.; Dutton, G.G.

European Journal of Biochemistry 213(1): 445-453

1993

ISSN/ISBN: 0014-2956
PMID: 8477716
DOI: 10.1111/j.1432-1033.1993.tb17780.x
Accession: 009465514
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The Klebsiella pneumoniae K40 capsular polysaccharide has been isolated and investigated by use of methylation analysis, specific degradations and NMR spectroscopy. The polysaccharide was depolymerised by a bacteriophage-associated endogalactosidase, and the resulting oligosaccharide was characterised by one-dimensional and two-dimensional NMR spectroscopy and direct chemical ionisation MS. The repeating unit of the K40 capsular polysaccharide was shown to be a linear hexasaccharide with the composition-->3)- alpha-L-Rhap-(1-->2)-alpha-L-Rhap-(1-->4)-alpha-D-GlcpA++ +-(1-->2-)- alpha-D-Manp-(1-->2)-alpha-D-Manp-(1-->3)-alpha-D-Galp-(1--> (Rha, rhamnose).

Structural determination of the capsular polysaccharide produced by Klebsiella pneumoniae serotype K40. NMR studies of the oligosaccharide obtained upon depolymerisation of the polysaccharide with a bacteriophage-associated endoglycanase

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Related References

Cescutti, P.; Toffanin, R.; Kvam, B.J.; Paoletti, S.; Dutton, G.G.S. 1993: Structural determnation of the capsular polysaccharide produced by Klebsiella pneumoniae serotype K40 : Nmr studies of the oligosaccharide obtained upon depolymerisation of the polysaccharide with a bacteriophage-associated endoglycanase FEBS Journal 213(1): 445-453
Cescutti, P.; Paoletti, S. 1994: On the specificity of a bacteriophage-borne endoglycanase for the native capsular polysaccharide produced by Klebsiella pneumoniae SK1 and its derived polymers Biochemical and Biophysical Research Communications 198(3): 1128-1134
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