+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Structure and dynamics of the sialic acid moiety of G-M3-ganglioside at the surface of a magnetically oriented membrane



Structure and dynamics of the sialic acid moiety of G-M3-ganglioside at the surface of a magnetically oriented membrane



Biochemistry 32(49): 13405-13413



1-3C-NMR techniques in oriented bilayer systems composed of DMPC and CHAPSO have been used to measure dipolar interactions between 13C-13C and 13C-1H pairs located in the sialic acid moiety of ganglioside G-M3. These interactions are reduced to a structural and motional model for the headgroup of this glycolipid using an order matrix approach. The analysis shows an average structure possessing a high degree of order and is most consistent with structures that are well extended from the membrane surface. Saturation of the ganglioside environment with high concentrations of Ca-2+ (0.28 M) produces a small perturbation of the head group. G-M3 dissolved in a DMPC/CHAPSO system can also be demonstrated to bind wheat germ agglutinin (WGA). The minimal perturbation of structure-dependent parameters suggests that the dominant structure of the sialic acid moiety in the isolated membrane system is favored for binding by the protein.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009467388

Download citation: RISBibTeXText

PMID: 8257677

DOI: 10.1021/bi00212a005


Related references

Structure and dynamics of sialic acid at the surface of a magnetically oriented membrane system. Biochemistry 32(13): 3422-3428, 1993

First synthesis of a pentasaccharide moiety of ganglioside GAA-7 containing unusually modified sialic acids through the use of N-Troc-sialic acid derivative as a key unit. Organic Letters 14(24): 6342-6345, 2013

Preparation of radioactive Tay-Sachs ganglioside labeled in the sialic acid moiety. Journal of Lipid Research 11(2): 144-149, 1970

Synthesis of the glycan moiety of ganglioside HPG-7 with an unusual trimer of sialic acid as the inner sugar residue. Chemical Communications 47(34): 9726-9728, 2011

Second generation mimics of ganglioside GM1 as artificial receptors for cholera toxin: Replacement of the sialic acid moiety. Bioorganic & Medicinal Chemistry Letters 10(19): 2197-2200, 2 October, 2000

A single site in human beta-hexosaminidase A binds both 6-sulfate-groups on hexosamines and the sialic acid moiety of GM2 ganglioside. Biochimica et Biophysica Acta 1637(1): 113-118, 2003

Muco lipidosis iv deficiency of ganglioside neuraminidase activity toward sialic acid galactose but not sialic acid sialic acid linkages. Pediatric Research 16(4 PART 2): 189A, 1982

The role of target membrane sialic acid residues in the fusion activity of the influenza virus: the effect of two types of ganglioside on the kinetics of membrane merging. Cellular & Molecular Biology Letters 9(2): 337-351, 2004

Structure of the ceramide moiety of GM1 ganglioside determines its occurrence in different detergent-resistant membrane domains in HL-60 cells. Biochemistry 42(21): 6608-6619, 2003

The effect of cold stress on ganglioside fatty acid composition and ganglioside-bound sialic acid content of rat brain subcellular fractions. Journal of Thermal Biology 26(3): 215-222, June, 2001

Molecular dynamics study of the conformations of glycosidic linkages in sialic acid modified ganglioside GM3 analogues. Glycoconjugate Journal 31(5): 365-386, 2015

Protein composition and structure of human placental microvillous membrane. External surface, sialic acid containing, extrinsic and intrinsic components. Experimental Cell Research 123(1): 167-176, 1979