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Structure and dynamics of the sialic acid moiety of GM3-ganglioside at the surface of a magnetically oriented membrane

Structure and dynamics of the sialic acid moiety of GM3-ganglioside at the surface of a magnetically oriented membrane

Biochemistry 32(49): 13405-13413

ISSN/ISBN: 0006-2960

PMID: 8257677

DOI: 10.1021/bi00212a005

1-3C-NMR techniques in oriented bilayer systems composed of DMPC and CHAPSO have been used to measure dipolar interactions between 13C-13C and 13C-1H pairs located in the sialic acid moiety of ganglioside G-M3. These interactions are reduced to a structural and motional model for the headgroup of this glycolipid using an order matrix approach. The analysis shows an average structure possessing a high degree of order and is most consistent with structures that are well extended from the membrane surface. Saturation of the ganglioside environment with high concentrations of Ca-2+ (0.28 M) produces a small perturbation of the head group. G-M3 dissolved in a DMPC/CHAPSO system can also be demonstrated to bind wheat germ agglutinin (WGA). The minimal perturbation of structure-dependent parameters suggests that the dominant structure of the sialic acid moiety in the isolated membrane system is favored for binding by the protein.

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Accession: 009467388

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