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Studies on the selections, the cross-resistance and the esterase activity in the strain of German cockroach, Blattella germanica L. selected with dichlorvos



Studies on the selections, the cross-resistance and the esterase activity in the strain of German cockroach, Blattella germanica L. selected with dichlorvos



Korean Journal of Applied Entomology 33(2): 74-80



The german cockroach, Blattella germanica L. populations were successively selected with dichlorvos for 11 generations. The resulting selected strain was investigated the resistance development, the cross resistance and the esterase activity. In the dichlorvos-selected(Rd) strain, the values of LC-50 increased 8.58 times more compared to the susceptible (S) strain. In the dichlorvos-selected (Rd) strains, the cross-resistance to chlorpyrifos, propoxur, fenvalerate and permethrin showed 3.35, 4.09, 2.83 and 2.00 times. Esterase-activity of the Rd strain showed 1.33 times higher than that of the S strain in the filter paper test. In comparison of zymogram patterns of the esterase isozyme by thin agarose gel electrophoresis against the german cockroach, the S strain was separated by 4 bands of esterase 3, 5, 6 and 8 bands and the Rd strain was separated by 6 bands of esterase 1, 2, 4, 5, 7 and 8 bands, and the resistant mechanisms of the Rd strain were considered as the 4 bands of esterase-1.2.4 and 7 bands except the common 2 bands of esterase-6 and 8 bands.

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