+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Subgenomic RNA synthesis directed by a synthetic defective interfering RNA of mouse hepatitis virus: a study of coronavirus transcription initiation



Subgenomic RNA synthesis directed by a synthetic defective interfering RNA of mouse hepatitis virus: a study of coronavirus transcription initiation



Journal of Virology 68(6): 3656-3666



We have used a full-length cDNA clone of a mouse hepatitis virus strain A59 defective interfering (DI) RNA, pMIDI-C, and cassette mutagenesis to study the mechanism of coronavirus subgenomic mRNA synthesis. Promoter sequences closely resembling those of subgenomic mRNAs 3 and 7 were inserted into MIDI-C. Both subgenomic RNA promoters gave rise to the synthesis of a subgenomic DI RNA in virus-infected and DI RNA-transfected cells. From a mutagenic analysis of the promoters we concluded the following. (i) The extent of base pairing between the leader RNA and the intergenic promoter sequence does not control subgenomic RNA abundance. (ii) Promoter recognition does not rely on base pairing only. Presumably, transcription initiation requires recognition of the promoter sequence by the transcriptase. (iii) Fusion of leader and body sequences takes place at multiple-possibly random-site; within the intergenic promoter sequence. A model is presented in which, prior to elongation, the leader RNA is trimmed by a processive 3' fwdarw 5' nuclease.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 009483836

Download citation: RISBibTeXText

PMID: 8189503


Related references

A subgenomic mRNA transcript of the coronavirus mouse hepatitis virus strain A59 defective interfering (DI) RNA is packaged when it contains the DI packaging signal. Journal of Virology 71(7): 5684-5687, 1997

Replication of synthetic defective interfering RNAs derived from coronavirus mouse hepatitis virus-A59. Virology 216(1): 174-183, 1996

Coronavirus transcription: subgenomic mouse hepatitis virus replicative intermediates function in RNA synthesis. Journal of Virology 64(3): 1050-1056, 1990

The 3' untranslated region of coronavirus RNA is required for subgenomic mRNA transcription from a defective interfering RNA. Journal of Virology 70(10): 7236-7240, 1996

Identification of a noncanonical signal for transcription of a novel subgenomic mRNA of mouse hepatitis virus: implication for the mechanism of coronavirus RNA transcription. Virology 278(1): 75-85, 2000

The leader RNA of coronavirus mouse hepatitis virus contains an enhancer-like element for subgenomic mRNA transcription. Journal of Virology 74(22): 10571-10580, 2000

Identification of a noncanonical transcription initiation site for transcription of a subgenomic mRNA of mouse hepatitis virus. Advances in Experimental Medicine and Biology 494: 563-570, 2001

Expression of hemagglutinin/esterase by a mouse hepatitis virus coronavirus defective-interfering RNA alters viral pathogenesis. Virology 242(1): 170-183, 1998

Translation but not the encoded sequence is essential for the efficient propagation of the defective interfering RNAs of the coronavirus mouse hepatitis virus. Journal of Virology 69(6): 3744-3751, 1995

Coronavirus defective-interfering RNA as an expression vector: the generation of a pseudorecombinant mouse hepatitis virus expressing hemagglutinin-esterase. Virology 208(1): 319-327, 1995

A system for study of coronavirus mRNA synthesis: a regulated, expressed subgenomic defective interfering RNA results from intergenic site insertion. Journal of Virology 65(11): 6031-6041, 1991

Replication of vesicular stomatitis virus defective interfering particle rna in vitro transition from synthesis of defective interfering leader rna to synthesis of full length defective interfering rna. Journal of Virology 46(2): 513-522, 1983

Synthesis of subgenomic mRNA's of mouse hepatitis virus is initiated independently: evidence from UV transcription mapping. Journal of Virology 39(2): 401-406, 1981

Inhibition of hepatitis C virus translation and subgenomic replication by small interfering RNAs directed against cellular RNA binding proteins. Journal of Hepatology 40(Suppl 1): 21, 2004

Mechanism of coronavirus transcription: duration of primary transcription initiation activity and effects of subgenomic RNA transcription on RNA replication. Journal of Virology 66(6): 3339-3346, 1992