EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Synoviocytes from osteoarthritis and rheumatoid arthritis produce plasminogen activators and plasminogen activator inhibitor-1 and display u-PA receptors on their surface



Synoviocytes from osteoarthritis and rheumatoid arthritis produce plasminogen activators and plasminogen activator inhibitor-1 and display u-PA receptors on their surface



Life Sciences 63(6): 441-453, July 3



The production of plasminogen activators and their inhibitors was studied in vitro in osteoarthritic (OA) and rheumatoid arthritic (RA) synovial fibroblasts (SF), obtained from RA and OA patients undergoing joint surgery. Subcultured SF were cultivated for 2, 4, 6, 8, 10 and 13 days and the medium assayed for the presence of both plasminogen activators (PAs) and plasminogen activator inhibitor-1 (PAI-1). The presence of urokinase-Plasminogen Activator (u-PA) receptors (u-PAR) on the surface of synovial cells was investigated by radio-ligand binding assay and crosslinking and by transmission electron microscopy (TEM) of a gold-u-PA complex. Our results showed a low production of tissue-type- Plasminogen Activator (t-PA) in both OA and RA SF, but relatively high levels of u-PA, until confluence, both in OA and in RA. SF were also able to produce plasminogen activator inhibitor in large amounts, in particular in RA since the very beginning of the culture. Receptors for u-PA were evident on both RA and OA SF. Our data show that SF in vitro produce mainly u-PA, the most important plasminogen activator involved in tissue modifications. The demonstration of u-PA receptors on the surface of OA and RA SF represents a step forward in the understanding of the possible role of fibrinolytic and tissue destructive proteinase cascade in joint inflammation.

(PDF emailed within 0-6 h: $19.90)

Accession: 009502851

Download citation: RISBibTeXText

PMID: 9718068

DOI: 10.1016/s0024-3205(98)00293-8



Related references

Difference in expression of plasminogen activators and plasminogen activator inhibitors in synovial tissue of patients with rheumatoid arthritis and osteoarthritis. Fibrinolysis 8(SUPPL 1): 146, 1994

Interleukin-1 beta, plasminogen activator and inhibitor of plasminogen activator in synovial fluid of rheumatoid arthritis, psoriatic arthritis and osteoarthritis. Agents and Actions 41(1-2): 88-89, 1994

Human ovarian surface epithelial cells can produce tissue-type plasminogen activator and plasminogen activator inhibitor-1. Japanese Journal of Fertility & Sterility 44(2): 147-152, April, 1999

Tissue plasminogen activator, plasminogen activator inhibitor-1 and von Willebrand factor rheumatoid arthritis. Clinical Rheumatology 12(3): 318-324, 1993

Plasminogen activators, plasminogen activator inhibitor type-1, steroidogenic acute regulatory protein, LH, and FSH receptors in corpus luteum of rhesus monkey. Biology of Reproduction 66(Supplement 1): 123, 2002

Gingival crevicular fluid tissue/blood vessel-type plasminogen activator and plasminogen activator inhibitor-2 levels in patients with rheumatoid arthritis: effects of nonsurgical periodontal therapy. Journal of Periodontal Research 52(3): 574-581, 2016

Human endothelial cells produce a plasminogen activator inhibitor and a tissue-type plasminogen activator-inhibitor complex. Biochimica et Biophysica Acta 802(1): 99-110, 1984

Plasminogen activators and plasminogen activator inhibitors in synovial fluid. Difference between inflammatory joint disorders and osteoarthritis. Journal of Rheumatology 20(1): 91-96, 1993

Urokinase-type plasminogen activator/type-2 plasminogen-activator inhibitor complexes are not internalized upon binding to the urokinase-type-plasminogen-activator receptor in THP-1 cells: Interaction of urokinase-type plasminogen activator/type-2 plasminogen-activator inhibitor complexes with the cell surface. European Journal of Biochemistry 233(2): 514-519, 1995

Gonadotropin surge-induced up-regulation of the plasminogen activators (tissue plasminogen activator and urokinase plasminogen activator) and the urokinase plasminogen activator receptor within bovine periovulatory follicular and luteal tissue. Biology of Reproduction 66(5): 1413-1421, 2002

Plasminogen activators and plasminogen activator inhibitor before and after surgery in patients with and without gastric malignancy. Haemostasis 25(5): 248-256, 1995

Plasminogen activators and plasminogen activator inhibitor-type-1 in rhesus monkey ovary. Biology of Reproduction 66(Supplement 1): 128-129, 2002