Synthesis, characterization, kinetic parameters, and diagnostic application of a sensitive colorimetric substrate for beta-galactosidase (2-chloro-4-nitrophenyl-beta-D-galactopyranoside)
Hwang, D., R.; Scott, M.E.
Bioorganic Chemistry 21(3): 284-293
1993
ISSN/ISBN: 0045-2068 Accession: 009510657
The synthesis and characterization of 2-chloro-4-nitrophenyl beta-D-galactopyranoside, an improved chromogenic substrate for beta-galactosidase, is described. The important kinetic parameters (K-m, V-max and K-p) for this substrate were compared with those of other substrates. The diagnostic utility of this substrate in a digoxin liposome immunoassay is discussed.
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Related References
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