+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

The Fc segment of IgE influences the kinetics of dissociation of a symmetrical bivalent ligand from cyclic dimeric complexes

The Fc segment of IgE influences the kinetics of dissociation of a symmetrical bivalent ligand from cyclic dimeric complexes

Biochemistry 35(17): 5518-5527

ISSN/ISBN: 0006-2960

PMID: 8611543

DOI: 10.1021/bi9523522

As part of a systematic effort to determine the features of immunoglobulin E-receptor (IgE-Fc epsilon RI) aggregation that are critical for cellular activation, we used fluorescence to examine the dissociation of a soluble bivalent ligand, N, N'-bis[[epsilon-[(2,4-dinitrophenyl)amino]caproyl]-L-tyrosyl]-L-cystine ((DCT)2-cys), from soluble bivalent IgE and its bivalent F(ab')2 and monovalent Fab' fragments. Cross-linking of Fab' fragments by (DCT)2-cys is limited to linear dimers, and we find that (DCT)2-cys dissociation from Fab' occurs with a single kinetic coefficient [(4.2 +/- 0.6) x 10-3 s-1] that corresponds to the lower of the two kinetic coefficients observed with the bivalent IgE [(4.7 +/- 0.7) x 10-2 s-1 and (4.4 +/- 0.3) x 10-3 s-1]. Similarly, the lower value is obtained for dissociation of (DCT)2-cys that is monovalently bound to IgE after incubation with a large excess of the ligand. (DCT)2-cys can bind to bivalent F(ab')2 fragments and form a variety of linear and cyclic aggregates, similarly to IgE, but, unlike IgE, we find that dissociation occurs with a single kinetic coefficient similar to that observed for Fab'. We find that IgE and its (Fab')2 fragments form highly stable cyclic dimer rings with two (DCT)2-cys. We demonstrate that the kinetic coefficients are independent of enhanced fluorescence quenching observed for bound sites in cyclic dimers. Together, the results show that the rate constant for breaking a linear cross-link formed by (DCT)2-cys is the same as that for dissociation of the monovalently bound (DCT)2-cys. Further, they show that opening of a bond in a dimer ring for the F(ab')2 fragment occurs with approximately the same dissociation rate constant as opening a bond in a linear cross-link. This rate constant is about three times smaller than that observed with IgE, suggesting that steric strain is caused by apposed Fc segments in cyclic IgE dimers. Such structural interference may affect the functional consequences of IgE-Fc epsilon RI aggregation on the cell surface.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 009532691

Download citation: RISBibTeXText

Related references

Formation of cyclic dimers of IgE-Fc-epsilon-RI complexes by a symmetrical bivalent ligand results in tyrosine phosphorylation of the gamma subunit but not the beta subunit of Fc-epsilon-RI and fails to activate p72-syk or cellular. FASEB Journal 10(6): A1215, 1996

Bivalent ligand dissociation kinetics from receptor-bound immunoglobulin E: evidence for a time-dependent increase in ligand rebinding at the cell surface. Biochemistry 30(9): 2357-2363, 1991

Bivalent ligand dissociation kinetics from receptor-bound immunoglobulin E : evidence for a time-dependence increase in ligand rebinding at the cell surface. Biochemistry (Easton) 30(9): 2357-2363, 1991

Dissociation kinetics of bivalent ligand-immunoglobulin E aggregates in solution. Biochemistry 30(9): 2348-2356, 1991

Kinetics of crosslinking anti-DNP IgE and anti-DNP Fab in solution by symmetrical bivalent ligand. Journal of Cellular Biochemistry Suppl 1994(18C): 246, 1994

Kinetics and mechanism of phosphine exchange for ruthenium(II) complexes in the series (η5-C5Me5)(PMe3)2RuX: ancillary ligand effects on dative ligand dissociation. Organometallics 8(2): 379-385, 1989

Synthesis, structure and spectral and redox properties of new mixed ligand monomeric and dimeric Ru(II) complexes: predominant formation of the "cis-alpha" diastereoisomer and unusual 3MC emission by dimeric complexes. Dalton Transactions 2006(5): 730-743, 2006

Stability and kinetics of decomposition of binuclear Cu(II) complexes with a symmetrical hexaaza macrocycle: the effect of SCN as ancillary ligand. Polyhedron 20(1-2): 75-82, 2001

The kinetics of bivalent ligand-bivalent receptor aggregation: Ring formation and the breakdown of the equivalent site approximation. Mathematical Biosciences 126(2): 171-190, 1995

Studies on the binding of a bivalent ligand to bivalent immunoglobulin e receptor complexes at the cell surface. FASEB Journal 5(4): A652, 1991

Kinetics of dissociation of copper(II) complexes with L-hydroxyproline and D-allohydroxyproline by cyclic voltammetry. Electrochimica Acta 36(1): 109-115, 1991

A dimeric IL-3 receptor agonist which exhibits characteristics of a bivalent ligand. Blood 86(10 Suppl 1): 24A, 1995

Kinetics of acid-catalysed dissociation of tetraaza-macrocyclic ligand complexes of copper(II). Journal of the Chemical Society. Dalton Transactions (2): 191-194, 1994

Kinetics of formation and dissociation of lanthanide(III) complexes with the 13-membered macrocyclic ligand TRITA4-. Dalton Transactions 2005(6): 1058-1065, 2005

Electron-capture dissociation and collision-induced dissociation of lanthanide metal-ligand complexes and lanthanide metal-ligand complexes bound to phosphopeptides. European Journal of Mass Spectrometry 15(2): 145-155, 2009