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The adsorption effects of a mineral adsorber of the clinoptilolite type part 1: Adsorption of aflatoxins B1 and G2



The adsorption effects of a mineral adsorber of the clinoptilolite type part 1: Adsorption of aflatoxins B1 and G2



Acta Veterinaria 44(5-6): 309-318



A new mineral adsorber, representing a concentrate of the zeolitic mineral clinoptilolite, was obtained by technological processing, from a zeolitic tuff deposit in Zlatokop (Serbia, Yugoslavia). In this paper, the adsorbing characteristics of this product for mycotoxins of the aflatoxin group (B1 and G2) are presented. The investigations were carried out at pH2 and pH7, in an electrolyte with a composition similar to animal gastric juice, in order to evaluate the influence of pH on the adsorptive characteristics of clinoptilolite. Mycotoxin determinations were carried out in the electrolyte without and with added mineral adsorber after incubation with shaking at 37 degree C (in a water bath). The test solution contained 1 g of mineral adsorber and certain concentrations of the toxins (50, 100, 200 and 300 mu-g/g). The quantity of mycotoxin not adsorbed was detected in the supernatant, after chloroform extraction, by the highly effective liquid chromatographic (HPLC) fluorescence method. Measurement of adsorption was started at 5 minutes and continued up to 48 hours of mycotoxin-mineral adsorber contact. The results obtained show that, immediately after contact with mineral adsorber (5 min) both mycotoxins were adsorbed more than 80%. The level of adsorption increased up to 48 hours without the occurrence of desorption. Mycotoxin B1 was better adsorbed from the neutral (pH7) than from the acidic (pH2) medium, while the mycotoxin G2 was better adsorbed in the acidic medium (pH2).

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