+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The antiglucocorticoid, RU486, attenuates stress-induced decreases in plasma-luteinizing hormone concentrations in male rats



The antiglucocorticoid, RU486, attenuates stress-induced decreases in plasma-luteinizing hormone concentrations in male rats



Neuroendocrinology 61(6): 638-645



The present studies investigated the role of glucocorticoid receptors (GR) in the inhibitory effects of acute and chronic immobilization stress on pituitary luteinizing hormone (LH) release. Systemic administration of the GR antagonist, RU486, significantly attenuated the acute decline in circulating LH observed in intact male rats immobilized by encasement within paper cocoons. Whereas vehicle-injected controls exhibited a significant reduction in plasma LH between +1 and +5 h of stress, animals given subcutaneous (sc) injections of 2.5 mg RU846/kg did not exhibit a reduction in circulating LH until 4 h after initiation of stress. Plasma LH levels in the GR-treated group were significantly elevated compared to the vehicle controls between + I and + 3 h of stress. Repetitive exposure to the same stress stimulus 24 and 48 h later resulted in decreased plasma LH levels in the vehicle-treated rats, but not in the animals injected sc with RU486. Other studies showed that intracerebroventricular (icv) administration of RU486 (10.0 mu-g/rat) also blunted the inhibitory effects of acute and chronic immobilization stress on pituitary LH release. In experiments designed to evaluate whether activation of central GR can influence the magnitude and/or temporal characteristics of the LH secretory response to acute inhibitory stress, it was observed that animals pretreated by icv injection of the GR agonist, RU362, exhibited a greater reduction in plasma LH levels during the first hour of stress, as compared to rats pretreated with vehicle alone. In summary, the present Findings that pharmacologic antagonism of GR attenuates acute and chronic stress-induced inhibition of circulating LH support a role for glucocorticoids in mechanisms underlying suppression of pituitary LH release during stress. The results also suggest that inhibitory glucocorticoid effects observed under these physiologic conditions may be mediated, in part, by activation of central GR. The present observations that exogenous stimulation of central GR potentiates the magnitude and onset of inhibitory patterns of LH release during acute stress support the hypothesis that GR-dependent neuroendocrine mechanisms are inhibitory to LH release during stress, and suggest that net suppression of circulating LH during stress may reflect the extent of GR occupation by endogenous glucocorticoids.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009542359

Download citation: RISBibTeXText

PMID: 7659189

DOI: 10.1159/000126890


Related references

Endogenous opiate involvement in acute and chronic stress induced changes in plasma luteinizing hormone concentrations in the male rat. Life Sciences 34(25): 2485-2494, 1984

Low dose testosterone induced suprastimulation of plasma luteinizing hormone levels in castrated adult male rats pulse characteristics of the plasma luteinizing hormone pattern. Biology of Reproduction 34(Suppl. 1): 115, 1986

Stress increases putative gonadotropin inhibitory hormone and decreases luteinizing hormone in male rats. Proceedings of the National Academy of Sciences of the United States of America 106(27): 11324-9, 2009

The effects of gonadal steroids and or castration on the stimulation of plasma luteinizing hormone induced by simultaneous administration of arginine vasotocin and luteinizing hormone releasing hormone in adult male rats. Anatomical Record 193(3): 709-710, 1979

Examination stress decreases plasma level of luteinizing hormone in male students. Psychosomatic Medicine 50(3): 286-294, 1988

Direct actions of the luteinizing hormone-releasing hormone agonist, deslorelin, on anterior pituitary contents of luteinizing hormone (LH) and follicle-stimulating hormone (FSH), LH and FSH subunit messenger ribonucleic acid, and plasma concentrations of LH and FSH in castrated male cattle. Biology of Reproduction 55(2): 386-392, 1996

The effects of luteinizing hormone releasing hormone infusion on plasma concentrations of luteinizing hormone testosterone and cortisol in the male. Acta Endocrinologica Supplementum 173: 92, 1973

Effect of stress on plasma levels of luteinizing hormone follicle stimulating hormone prolactin thyroid stimulating hormone and growth hormone in normal male rats. Federation Proceedings 32(3 Part 1): 281, 1973

Reversal by arginine-vasotocin of the effects of pinealectomy on the amount of 5-hydroxytryptamine in the hypothalamus and the concentrations of luteinizing hormone and follicle-stimulating hormone in the plasma of immature male rats. Journal of Endocrinology 84(1): 159-161, 1980

RU486 administration blocks neuropeptide Y potentiation of luteinizing hormone (LH)-releasing hormone-induced LH surges in proestrous rats. Endocrinology 133(6): 2418-2423, 1993

Plasma luteinizing hormone responses in hyper prolactinemic male rats to luteinizing hormone releasing hormone before and after bromocriptine treatment and castration. Acta Endocrinologica Suppl. (225): 141, 1979

Plasma luteinizing hormone response to luteinizing hormone releasing hormone injection in immature intact castrated and cyproterone treated male rats. Journal of Reproduction & Fertility 48(1): 195-197, 1976

Reversal by arginine vasotocin of the effects of pinealectomy on the amount of 5 hydroxy tryptamine in the hypothalamus and the concentrations of luteinizing hormone and follicle stimulating hormone and in the plasma of immature male rats. Journal of Endocrinology 84(1): 159-162, 1980

Enhancement of the plasma luteinizing hormone response to luteinizing hormone releasing hormone by arginine vasotocin in adult male rats. Anatomical Record 193(3): 651-652, 1979

Interaction of luteinizing hormone-releasing hormone, cyproterone acetate and arginine vasotocin on plasma levels of luteinizing hormone in intact and castrated adult male rats. Molecular and Cellular Endocrinology 14(1): 59-71, 1979