+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The distribution of inflammatory demyelinated lesions in the central nervous system of rats with antibody-augmented demyelinating experimental allergic encephalomyelitis

The distribution of inflammatory demyelinated lesions in the central nervous system of rats with antibody-augmented demyelinating experimental allergic encephalomyelitis

Experimental Neurology 129(2): 299-310

Experimental allergic encephalomyelitis (EAE) has long been studied as an animal model of the human demyelinating disease Multiple Sclerosis. However, EAE induced in the Lewis rat by injection of myelin basic protein (MBP), or MBP-specific T-lymphocytes, is primarily an inflammatory condition of the central nervous system (CNS) with little or no demyelination. In EAE models in which demyelination does result, it is either not very widespread or is unpredictable in its degree and location. In this study we have produced antibody-augmented demyelinating EAE (ADEAE) in the Lewis rat by injection of activated MBP-specific T-lymphoblasts, followed by injection 4 days later of a monoclonal antibody against myelin/oligodendrocyte glycoprotein, an extrinsic protein of myelin. We have documented the extent and location of inflammatory cell infiltrates and demyelination throughout the CNS using histochemistry, immunofluorescence, and image analysis. Perivascular inflammatory infiltrates were seen in the deep cerebellar white matter and in the folia. Perivascular, periventricular, and subpial inflammation was widespread throughout the pons/medulla and at all levels of the spinal cord. Very little inflammation was apparent in the forebrain. MBP immunofluorescence demonstrated extensive areas of periventricular demyelination in the forebrain around the third ventricle. Both periventricular and perivascular lesions were commonly observed in the cerebellum and pons/medulla. The extent of demyelination in the spinal cord increased caudally with large confluent areas of subpial demyelination seen throughout the lumbar cord. The extensive and reproducible distribution of inflammatory demyelinating lesions in ADEAE provide the possibility to select areas of the CNS for more detailed analysis of the cellular changes that accompany demyelination and remyelination.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 009556967

Download citation: RISBibTeXText

PMID: 7525334

DOI: 10.1006/exnr.1994.1172

Related references

Do neuroleptics prevent relapse? Clinical observations in a psychosocial rehabilitation program. Psychiatric Quarterly 58(1): 42-50, 1986

Experimental allergic encephalomyelitis: serum immunoglobulin binds to myelin and oligodendrocytes in cultured tissue: ultrastructural-immunoperoxidase observations. Laboratory Investigation; A Journal of Technical Methods and Pathology 40(5): 568-575, 1979

The distribution of minimal inhibitory concentrations of recently introduced cephalosporins in multiresistant strains of Escherichia coli, Proteus mirabilis and Klebsiella spec. as revealed by zone sizes of a standardized agar diffusion test. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie 245(3): 317-323, 1979

The peroxynitrite product 3-nitro-L-tyrosine attenuates the hemodynamic responses to angiotensin II in vivo. European Journal of Pharmacology 315(2): 165-170, 1996

Asparaginase in combination chemotherapy for remission induction of childhood acute lymphocytic leukemia. Cancer Research 33(9): 2020-2025, 1973

Adoptively transferred chronic relapsing experimental autoimmune encephalomyelitis in the mouse. Neuropathologic analysis. Laboratory Investigation; a Journal of Technical Methods and Pathology 51(5): 534-546, 1984

Adoption of children from countries with endemic hepatitis B. Pediatric Infectious Disease Journal 6(5): 431-437, 1987

Expression of CRABP-I and -II in human epidermal cells. Alteration of relative protein amounts is linked to the state of differentiation. Biochemical Journal 287: 383-389, 1992

Isolation and characterization of the immunizing agent of crystal violet vaccines against hog cholera. Archiv für die Gesamte Virusforschung 44(2): 133-141, 1974

Peripheral nervous system lesions in chronic renal insufficiency. Klinicheskaia Meditsina 57(9): 12-16, 1979

MRNA expression, measured by quantitative reverse transcriptase polymerase chain reaction, of five putative drug resistance parameters, in normal and leukaemic peripheral blood and bone marrow. Acta Haematologica 98(1): 1-7, 1997

The distribution of immunoglobulin containing cells in the human inferior nasal turbinate in relation to nasal allergy. Practica Otologica Kyoto 78(5): 731-747, 1985

Enzymatic degradation of linseed mucilage. I. Isolation and characterization of extracellular enzyme produced by Aspergillus niger. Biochimica et Biophysica Acta 167(3): 590-596, 1968

Fat control of milk and dairy products by gas chromatography. Hemika Hronika 39(8/9/10): 172-177, 1974

Microglia isolated from rat brain secrete a urokinase type plasminogen activator. Brain Research 577(2): 285-292, 1992